@article{c3fd9e40773f46948165b608a9ffc6da,
title = "An immune-responsive Serpin regulates the melanization cascade in Drosophila",
abstract = "In arthropods, the melanization reaction is associated with multiple host defense mechanism leading to the sequestration and killing of invading microorganisms. Arthropod melanization is controlled by a cascade of serine proteases that ultimately activates the enzyme prophenoloxidase (PPO), which, in turn, catalyzes the synthesis of melanin. Here we report the biochemical and genetic characterization of a Drosophila serine protease inhibitor protein, Serpin-27A, which regulates the melanization cascade through the specific inhibition of the terminal protease prophenoloxidase-activating enzyme. Our data demonstrate that Serpin-27A is required to restrict the phenoloxidase activity to the site of injury or infection, preventing the insect from excessive melanization.",
author = "{De Gregorio}, Ennio and Han, {Sung Jun} and Lee, {Won Jae} and Baek, {Min Ji} and Tsukasa Osaki and Kawabata, {Shun Ichiro} and Lee, {Bok Leul} and Sadaaki Iwanaga and Bruno Lemaitre and Brey, {Paul T.}",
note = "Funding Information: We thank Fran{\c c}ois Leulier, Nicolas Vodovar, Brigitte Maroni, Sylvie Perrot, and Charles Roth for assistance and stimulating discussions. E.D.G. was supported by a Human Frontier Science Program fellowship. The laboratory of B.L. was funded by the Association pour la Recherche contre le Cancer (ARC), the Fondation pour la Recherche M{\'e}dicale (FRM), and Programme Microbiologie (PRMMIP00). The laboratory of P.T.B. was supported by grants from the Institut Pasteur and the CNRS Programme International de Cooperation Scientifique N°907 France-Japon. S.-J.H. was the recipient of a French Government Doctoral Scholarship.",
year = "2002",
month = oct,
day = "1",
doi = "10.1016/S1534-5807(02)00267-8",
language = "English",
volume = "3",
pages = "581--592",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "4",
}