All-in-one uniified microfluidic chip for automation of embryonic cell manipulation

Shinya Sakuma, Yoko Yamanishi, Fumihito Arai, Tatsuo Arai, Akiyuki Hasegawa, Tamio Tanikawa, Akihiko Ichikawa, Osamu Satoh, Akihiro Nakayama, Hiroshi Aso, Mitsuhiro Goto, Seiya Takahashi, Kazutsugu Matsukawa

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

5 被引用数 (Scopus)

抄録

We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and they are fused by an electrical field. For the current study, the microfluidic chip was designed to have 3 parts for this process. Then, all-in-one unified microfluidic chip was successfully fabricated. Basic functions of these parts are verified independently.

本文言語英語
ホスト出版物のタイトルProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社Chemical and Biological Microsystems Society
ページ1883-1885
ページ数3
ISBN(印刷版)9780979806421
出版ステータス出版済み - 2009
外部発表はい
イベント13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, 韓国
継続期間: 11月 1 200911月 5 2009

出版物シリーズ

名前Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

その他

その他13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
国/地域韓国
CityJeju
Period11/1/0911/5/09

!!!All Science Journal Classification (ASJC) codes

  • 化学工学(その他)
  • バイオエンジニアリング

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