Adipose tissue complement factor B promotes adipocyte maturation

Hiroaki Matsunaga, Misaki Iwashita, Takanori Shinjo, Akiko Yamashita, Mitsudai Tsuruta, Shoichiro Nagasaka, Ataru Taniguchi, Mitsuo Fukushima, Naoya Watanabe, Fusanori Nishimura

研究成果: ジャーナルへの寄稿学術誌査読

20 被引用数 (Scopus)


Objectives It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. Methods We investigated the expression level of complement system-related genes in adipocytes. To understand the role of CfB in adipocyte, we performed Cfb overexpression in 3T3-L1 preadipocytes and generated adipocyte-specific Cfb transgenic mice. Results Cfb expression was markedly enhanced in 3T3-L1 adipocytes co-cultured with macrophages following endotoxin stimulation. In Cfb-overexpressing cells, the expression of adipocyte differentiation/maturation-related genes encoding peroxisome proliferator-activated receptor γ (Pparγ), adipocyte Protein 2 and perilipin was significantly enhanced. Cfb transgenic mice showed a marked increase in the expression of genes encoding Pparγ, perilipin, sterol regulatory element-binding protein 1 c, and Cd36 in the subcutaneous adipose tissue. Conclusions CfB plays a crucial role in late-phase of adipocyte differentiation and subsequent lipid droplet formation.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータス出版済み - 1月 1 2018

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


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