Adenine inhibits TNF-α signaling in intestinal epithelial cells and reduces mucosal inflammation in a dextran sodium sulfate-induced colitis mouse model

Toshihiko Fukuda, Kaustav Majumder, Hua Zhang, Patricia V. Turner, Toshiro Matsui, Yoshinori Mine

研究成果: ジャーナルへの寄稿学術誌査読

20 被引用数 (Scopus)

抄録

Adenine (6-amino-6H-purine), found in molokheiya (Corchorus olitorius L.), has exerted vasorelaxation effects in the thoracic aorta. However, the mode of action of the anti-inflammatory effect of adenine is unclear. Thus, we investigated to clarify the effect of adenine on chronic inflammation of the gastrointestinal tract. In intestinal epithelial cells, adenine significantly inhibited tumor necrosis factor-α-induced interleukin-8 secretion. The inhibition of adenine was abolished under the treatment of inhibitors of adenyl cyclase (AC) and protein kinase A (PKA), indicating the effect of adenine was mediated through the AC/PKA pathway. Adenine (5, 10, and 50 mg/kg BW/day) was administered orally for 14 days to female BALB/c mice, and then 5% dextran sodium sulfate (DSS) was given to induce colitis. Adenine (5 mg/kg BW/day) significantly prevented DSS-induced colon shortening, expression of pro-inflammatory cytokines, and histological damage in the colon. These results suggest that adenine can be a promising nutraceutical for the prevention of intestinal inflammation.

本文言語英語
ページ(範囲)4227-4234
ページ数8
ジャーナルJournal of Agricultural and Food Chemistry
64
21
DOI
出版ステータス出版済み - 6月 1 2016

!!!All Science Journal Classification (ASJC) codes

  • 化学一般
  • 農業および生物科学一般

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