Accumulation of p60lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171

Yoichi Moroi, Yasuhiro Koga, Kazuhiko Nakamura, Masumi Ohtsu, Genki Kimura, Kikuo Nomoto

研究成果: ジャーナルへの寄稿学術誌査読

22 被引用数 (Scopus)

抄録

The lck gene encodes a protein tyrosine kinase of nonreceptor type, p56lck, whose expression occurs almost exclusively in T lymphocytes. MOL 171, an anti‐p56lck monoclonal antibody, was produced by using a 25‐amino‐acid synthetic polypeptide as the antigen, its structure corresponding to the N‐terminal region deduced from the lck cDNA sequence. Immunoblot analysis with MOL 171 showed the accumulation of 60 kD form of Lck protein, p60lck, and the decrease of p56lck in human T cell leukemia virus type I (HTLV‐I)‐transformed T cell lines. Another anti‐Lck monoclonal antibody, MOL 294, raised by using a synthetic peptide corresponding to the C‐terminal region deduced from the lck cDNA sequence, also detected the accumulation of p60lck in those HTLV‐1‐transformed T cell lines. The appearance of p60lck with the decrease of p56lck in normal T lymphocytes after stimulation suggested the origin of p60lck in HTLV‐I‐transformed T cells.

本文言語英語
ページ(範囲)909-915
ページ数7
ジャーナルJapanese Journal of Cancer Research
82
8
DOI
出版ステータス出版済み - 8月 1991
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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