TY - JOUR
T1 - Accumulation of αB-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5′-flanking and coding sequence of the genomic DNA
AU - Iwaki, Akiko
AU - Iwaki, Toru
AU - Goldman, James E.
AU - Ogomori, Koji
AU - Tateishi, Jun
AU - Sakaki, Yoshiyuki
N1 - Funding Information:
The authors thank Drs. Berkely Powell and Melvin Ball for the Alexander's disease CNS tissue. This study was supported by a Grant-in-Aid for General Scientific Research fi'om the Ministry of Education, Science and Culture of Japan, by a Grant for Aging and Health from Ministry of Health and Welfare of Japan (T.I.) and by NIH Grants EY09331 and a Javits Neuroscience Investigator Award, NS17125, USA (J.E.G.).
PY - 1992/6/8
Y1 - 1992/6/8
N2 - αB-Crystallin is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease. To examine whether or not accumulation of αB-crystallin is due to any abnormality of the gene structures, we determined the sequence of the αB-crystallin gene in two cases of pathologically confirmed Alexander's disease. Direct sequencing of the promoter and coding regions of the αB-crystallin gene in patients revealed them to have a normal sequence. Northern blotting showed a single αB-crystallin mRNA species expressed in the Alexander's disease brain.
AB - αB-Crystallin is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease. To examine whether or not accumulation of αB-crystallin is due to any abnormality of the gene structures, we determined the sequence of the αB-crystallin gene in two cases of pathologically confirmed Alexander's disease. Direct sequencing of the promoter and coding regions of the αB-crystallin gene in patients revealed them to have a normal sequence. Northern blotting showed a single αB-crystallin mRNA species expressed in the Alexander's disease brain.
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U2 - 10.1016/0304-3940(92)90689-5
DO - 10.1016/0304-3940(92)90689-5
M3 - Article
C2 - 1407707
AN - SCOPUS:0026704154
SN - 0304-3940
VL - 140
SP - 89
EP - 92
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -