A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

Hirokazu Suzuki; Keisuke Wada; Megumi Furukawa; Katsumi Doi; Toshihisa Ohshima

doi:10.1271/bbb.130492

A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

Hirokazu Suzuki
, Keisuke Wada
, Megumi Furukawa
, Katsumi Doi
, Toshihisa Ohshima

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

16 被引用数 (Scopus)

抄録

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

本文言語	英語
ページ（範囲）	2316-2318
ページ数	3
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	77
号	11
DOI	https://doi.org/10.1271/bbb.130492
出版ステータス	出版済み - 2013
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

医学一般

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10.1271/bbb.130492

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title = "A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426",

abstract = "This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.",

author = "Hirokazu Suzuki and Keisuke Wada and Megumi Furukawa and Katsumi Doi and Toshihisa Ohshima",

note = "Funding Information: This work was supported by JSPS KAKENHI Grant no. 25450105, and by the Institute for Fermentation, Osaka, Japan.",

year = "2013",

doi = "10.1271/bbb.130492",

language = "English",

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T1 - A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

AU - Suzuki, Hirokazu

AU - Wada, Keisuke

AU - Furukawa, Megumi

AU - Doi, Katsumi

AU - Ohshima, Toshihisa

N1 - Funding Information: This work was supported by JSPS KAKENHI Grant no. 25450105, and by the Institute for Fermentation, Osaka, Japan.

PY - 2013

Y1 - 2013

N2 - This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

AB - This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

UR - https://www.scopus.com/pages/publications/84888867358

UR - https://www.scopus.com/pages/publications/84888867358#tab=citedBy

U2 - 10.1271/bbb.130492

DO - 10.1271/bbb.130492

M3 - Article

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AN - SCOPUS:84888867358

SN - 0916-8451

VL - 77

SP - 2316

EP - 2318

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 11

ER -

A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

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!!!All Science Journal Classification (ASJC) codes

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