TY - GEN
T1 - A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane
AU - Kaji, Noritada
AU - Koyama, Ryo
AU - Yasui, Takao
AU - Higashiyama, Tetsuya
AU - Baba, Yoshinobu
N1 - Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - In this study, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.
AB - In this study, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.
UR - http://www.scopus.com/inward/record.url?scp=84983356231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983356231&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983356231
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 832
EP - 834
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -