TY - JOUR
T1 - A novel metabolic communication between neurons and astrocytes
T2 - Non-essential amino acid L-serine released from astrocytes is essential for developing hippocampal neurons
AU - Mitoma, Junya
AU - Furuya, Shigeki
AU - Hirabayashi, Yoshio
N1 - Funding Information:
We thank Dr K. Takio and Mr M. Chijimatsu (Division of Biomolecular Characterization, RIKEN) for the amino acid analysis. We also thank Dr R.T. Kado (Centre National de la Recherche Scientifique, Gif sur Yvette) for his critical reading of the manuscript. We are grateful to Professor Y. Nagai (Mitsubishi Kagaku Institute of Life Science and RIKEN) for his encouragement. This work was supported by the Special Postdoctoral Researchers Program, Frontier Research Program of the Institute of Physical and Chemical Research (RIKEN), and the Grants-in-Aid for Encouragement of Young Scientists (No. 09780671 to J.M.) from the Ministry of Education, Science and Culture of Japan.
PY - 1998/2
Y1 - 1998/2
N2 - A hippocampal astrocyte conditioned medium (HACM) supported the survival of hippocampal neurons under a serum-, glia-free culture setting. The neurotrophic activity in HACM was mostly recovered in low molecular weight fractions (M(r) < 3000), which contained high levels of L-serine and L-alanine. However, L-serine alone significantly improved the neuronal survival and neurite growth in a stereo-specific manner. Other non-essential amino acids had no effect. These results strongly suggest that L-serine, released by astrocytes, is essential for the survival and phenotypic growth of hippocampal neurons.
AB - A hippocampal astrocyte conditioned medium (HACM) supported the survival of hippocampal neurons under a serum-, glia-free culture setting. The neurotrophic activity in HACM was mostly recovered in low molecular weight fractions (M(r) < 3000), which contained high levels of L-serine and L-alanine. However, L-serine alone significantly improved the neuronal survival and neurite growth in a stereo-specific manner. Other non-essential amino acids had no effect. These results strongly suggest that L-serine, released by astrocytes, is essential for the survival and phenotypic growth of hippocampal neurons.
UR - https://www.scopus.com/pages/publications/0031931051
UR - https://www.scopus.com/pages/publications/0031931051#tab=citedBy
U2 - 10.1016/S0168-0102(97)00113-2
DO - 10.1016/S0168-0102(97)00113-2
M3 - Article
C2 - 9579653
AN - SCOPUS:0031931051
SN - 0168-0102
VL - 30
SP - 195
EP - 199
JO - Neuroscience Research
JF - Neuroscience Research
IS - 2
ER -