A centrifugation-assisted peptide-mediated gene transfer method for high-throughput analyses

Mitsuhiro Kimura, Takeshi Yoshizumi, Keiji Numata

研究成果: ジャーナルへの寄稿コメント/討論査読

3 被引用数 (Scopus)

抄録

A peptide-mediated DNA delivery system for several plant species has been recently developed. This system uses ionic complexes of DNA and fusion peptides containing several domains, such as DNA-binding and cell-penetrating peptides. Although the peptide-DNA complexes are capable of penetrating into plant cells through the cell wall by mechanical pressure using a syringe, sample throughput is limited. Here, we describe a Centrifugation-Assisted Peptide-mediated gene Transfer (CAPT) method for improving sample throughput with reproducible gene transfer efficiency. We optimized the parameters of CAPT for transient gene transfer efficiency by using Nicotiana tabacum cotyledons as a model plant material. The optimal parameters for centrifugation were 10,000×g for 60 s. Furthermore, we successfully transferred the peptide–DNA complex into rice cotyledons using the optimized CAPT method. Thus, the CAPT method is superior to the previous syringe-mediated infiltration method in terms of sample throughput in multiple plant species.

本文言語英語
ページ(範囲)49-52
ページ数4
ジャーナルPlant Biotechnology
36
1
DOI
出版ステータス出版済み - 2019
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 農業および作物学
  • 植物科学

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