ウシ血清アルブミン固定化シートを用いたエンドウの根滲出物の可視化

Takeshi Onodera, Haruna Miyazaki, Masaru Nakayasu, Nozomu Sakurai, Akifumi Sugiyama

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

In leguminous plants, rhizobium symbiotically forms nodules, which are responsible for nitrogen fixation and obtaining nutrients necessary for growth. Plant roots are thought to secrete chemicals that signal the formation of nodules. In this study, we attempted to visualize the concentration distribution of the exudates from pea roots by using the phenomenon that the fluorescence intensity decreases due to the interaction of bovine serum albumin (BSA) with phenolic compounds. BSA was immobilized on a glass microfiber filter (sheets with immobilized BSA), and the quenched fluorescence of BSA due to the interaction with root exudates was visualized. The correlation coefficient between the quenched fluorescence intensity per sheet and the amount of kaempferol in hydrolyzed sample recovered from six sheets with immobilized BSA was 0.8 (p < 0.05), indicating a high correlation.

寄稿の翻訳タイトルVisualization of Root Exudates from Pea Roots Using Sheets with Immobilized Bovine Serum Albumin
本文言語日本語
ページ(範囲)133-138
ページ数6
ジャーナルIEEJ Transactions on Sensors and Micromachines
142
7
DOI
出版ステータス出版済み - 2022

!!!All Science Journal Classification (ASJC) codes

  • 機械工学
  • 電子工学および電気工学

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