TY - JOUR
T1 - VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for golgi and ER assembly in vivo
AU - Uchiyama, Keiji
AU - Jokitalo, Eija
AU - Kano, Fumi
AU - Murata, Masayuki
AU - Zhang, Xiaodong
AU - Canas, Benito
AU - Newman, Richard
AU - Rabouille, Catherine
AU - Pappin, Darryl
AU - Freemont, Paul
AU - Kondo, Hisao
PY - 2002/12/9
Y1 - 2002/12/9
N2 - NSF and p97 are ATPases required for the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of organelles. NSF uses ATP hydrolysis to dissociate NSF/SNAPs/SNAREs complexes, separating the v- and t-SNAREs, which are then primed for subsequent rounds of fusion. In contrast, p97 does not dissociate the p97/p47/SNARE complex even in the presence of ATP. Now we have identified a novel essential factor for p97/p47-mediated membrane fusion, named VCIP135 (valosin-containing protein [VCP][p97]/p47 complex-interacting protein, p135), and show that it binds to the p97/p47/syntaxin5 complex and dissociates it via p97 catalyzed ATP hydrolysis. In living cells, VCIP135 and p47 are shown to function in Golgi and ER assembly.
AB - NSF and p97 are ATPases required for the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of organelles. NSF uses ATP hydrolysis to dissociate NSF/SNAPs/SNAREs complexes, separating the v- and t-SNAREs, which are then primed for subsequent rounds of fusion. In contrast, p97 does not dissociate the p97/p47/SNARE complex even in the presence of ATP. Now we have identified a novel essential factor for p97/p47-mediated membrane fusion, named VCIP135 (valosin-containing protein [VCP][p97]/p47 complex-interacting protein, p135), and show that it binds to the p97/p47/syntaxin5 complex and dissociates it via p97 catalyzed ATP hydrolysis. In living cells, VCIP135 and p47 are shown to function in Golgi and ER assembly.
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U2 - 10.1083/jcb.200208112
DO - 10.1083/jcb.200208112
M3 - Article
C2 - 12473691
AN - SCOPUS:0037049466
SN - 0021-9525
VL - 159
SP - 855
EP - 866
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -