TY - JOUR
T1 - Variability in microbial communities in black smoker chimneys at the NW caldera vent field, brothers volcano, Kermadec arc
AU - Takai, Ken
AU - Nunoura, Takuro
AU - Horikoshi, Koki
AU - Shibuya, Takazo
AU - Nakamura, Kentaro
AU - Suzuki, Yohey
AU - Stott, Matthew
AU - Massoth, Gary J.
AU - Christenson, B. W.
AU - deRonde, Cornel E.J.
AU - Butterfield, David A.
AU - Ishibashi, Jun ichiro
AU - Lupton, John E.
AU - Evans, L. J.
N1 - Funding Information:
We are grateful to the captain and crew of the R/V Yokosuka and the operation team of the DSV Shinkai 6500 for their technical expertise and safety at sea. We wish to thank onboard scientists of the SWEEP VENTS cruise for their support. This work was supported in part by Grant-in-Aid for Science Research (Nos. 15403014 and 16340173) from MEXT, Japan, and by New Zealand Foundation for Research, Science and Technology (FRST) grant C05X0406.
PY - 2009/12
Y1 - 2009/12
N2 - Microbial communities in black smoker chimney structures at the NW caldera vent field of the Brothers volcano, Kermadec arc were characterized by using both culture-dependent and - independent techniques. The hydrothermal vent fluid chemistry, as given by end-member salinities and gas contents, differ among the black smoker sites of the NW caldera field, indicating probable phase-separation-controlled variability in the fluid chemistry. Chimney structures collected from typical Cl-depleted and Clenriched hydrothermal fluid vents were used for the microbiological investigation. The 16S rRNA gene clone analysis showed that the archaeal rRNA gene communities were similar within interior and exterior substructures of any single chimneys, and even between chimneys having either Cl-depleted or Cl-enriched hydrothermal emissions. By contrast, the bacterial rRNA gene communities varied between chimneys hosting Cl-depleted or Clenriched fluids. Cultivation analysis showed significant variation in the viable counts of various microbial components among the chimneys, particularly of H2 - and/or S-oxidizing chemolithotrophs such as the genera Persephonella and Sulfurimonas. The difference shown by the cultured microbial community structures between the chimneys may be related to the different chemistries of hydrothermal fluids being expelled by the chimney structures, and possibly differences in the subseafloor environments beneath the vent sites, especially when considering different gas inputs and carbon sources. The patterns in cultivated microbial populations in the chimney structures were compared among the chimney structures studied so far from various deep-sea hydrothermal fields including this study. It shows that the patterns from the gas-rich hydrothermal fluid chimneys are quite similar between the geographically and geologically different hydrothermal fields of the Brothers NW caldera vent field, and the Mariner vent field of the Valu Fa Ridge.
AB - Microbial communities in black smoker chimney structures at the NW caldera vent field of the Brothers volcano, Kermadec arc were characterized by using both culture-dependent and - independent techniques. The hydrothermal vent fluid chemistry, as given by end-member salinities and gas contents, differ among the black smoker sites of the NW caldera field, indicating probable phase-separation-controlled variability in the fluid chemistry. Chimney structures collected from typical Cl-depleted and Clenriched hydrothermal fluid vents were used for the microbiological investigation. The 16S rRNA gene clone analysis showed that the archaeal rRNA gene communities were similar within interior and exterior substructures of any single chimneys, and even between chimneys having either Cl-depleted or Cl-enriched hydrothermal emissions. By contrast, the bacterial rRNA gene communities varied between chimneys hosting Cl-depleted or Clenriched fluids. Cultivation analysis showed significant variation in the viable counts of various microbial components among the chimneys, particularly of H2 - and/or S-oxidizing chemolithotrophs such as the genera Persephonella and Sulfurimonas. The difference shown by the cultured microbial community structures between the chimneys may be related to the different chemistries of hydrothermal fluids being expelled by the chimney structures, and possibly differences in the subseafloor environments beneath the vent sites, especially when considering different gas inputs and carbon sources. The patterns in cultivated microbial populations in the chimney structures were compared among the chimney structures studied so far from various deep-sea hydrothermal fields including this study. It shows that the patterns from the gas-rich hydrothermal fluid chimneys are quite similar between the geographically and geologically different hydrothermal fields of the Brothers NW caldera vent field, and the Mariner vent field of the Valu Fa Ridge.
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U2 - 10.1080/01490450903304949
DO - 10.1080/01490450903304949
M3 - Article
AN - SCOPUS:71149094725
SN - 0149-0451
VL - 26
SP - 252
EP - 269
JO - Geomicrobiology Journal
JF - Geomicrobiology Journal
IS - 8
ER -