Utilization of fermented barley extract derived from a by-product of barley shochu for a microbial medium-note-

Fermented barley extract (FBE) obtained from a barley shochu by-product (shochu kasu) was evaluated as a nitrogen source for a medium of Lactobacillus fermentum NBRC 3071, Lactococcus lactis subsp. lactis ATCC 11454, Enterococcus faecalis NCIMB 8275, and Bifidobacterium longum JCM 1217^T, by comparing it with a nutritionally rich laboratory-use nitrogen source (yeast extract, polypeptone, casein, and meat extract) in the basal medium at the same level of total nitrogen (TN). The FBE medium yielded higher rates and maxima of cell growth of L. fermentum NBRC 3071 and B. longum JCM 1217^T than the basal medium. On the other hand, the FBE medium yielded slightly lower rates of cell growth of L. lactis subsp. lactis ATCC 11454 and E. faecalis NCIMB 8275 than the basal medium. However, despite the inhibitory effect in the initial phase, the final cell growth level in the FBE medium was the same as that in the basal medium.