Using saturated absorption for superresolution laser scanning transmission microscopy

Kentaro Nishida; Hikaru Sato; Ryosuke Oketani; Kentaro Mochizuki; Kenta Temma; Yasuaki Kumamoto; Hideo Tanaka; Katsumasa Fujita

doi:10.1111/jmi.13033

Using saturated absorption for superresolution laser scanning transmission microscopy

Kentaro Nishida, Hikaru Sato, Ryosuke Oketani, Kentaro Mochizuki, Kenta Temma, Yasuaki Kumamoto, Hideo Tanaka, Katsumasa Fujita

Research output: Contribution to journal › Article › peer-review

Abstract

We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.

Original language	English
Pages (from-to)	117-129
Number of pages	13
Journal	Journal of Microscopy
Volume	288
Issue number	2
DOIs	https://doi.org/10.1111/jmi.13033
Publication status	Published - Nov 2022
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Histology

Access to Document

10.1111/jmi.13033

Cite this

@article{062da90f4f414fca85d4497e0e9409e0,

title = "Using saturated absorption for superresolution laser scanning transmission microscopy",

abstract = "We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.",

author = "Kentaro Nishida and Hikaru Sato and Ryosuke Oketani and Kentaro Mochizuki and Kenta Temma and Yasuaki Kumamoto and Hideo Tanaka and Katsumasa Fujita",

note = "Funding Information: This work was supported by JST CREST and COI-NEXT Grant No. JPMJCR15N3 and JPMJPF2009, Japan. The authors thank T. Kubo of Osaka University for his valuable advice. Funding Information: This work was supported by JST CREST and COI‐NEXT Grant No. JPMJCR15N3 and JPMJPF2009, Japan. The authors thank T. Kubo of Osaka University for his valuable advice. Publisher Copyright: {\textcopyright} 2021 Royal Microscopical Society.",

year = "2022",

month = nov,

doi = "10.1111/jmi.13033",

language = "English",

volume = "288",

pages = "117--129",

journal = "Journal of Microscopy",

issn = "0022-2720",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Using saturated absorption for superresolution laser scanning transmission microscopy

AU - Nishida, Kentaro

AU - Sato, Hikaru

AU - Oketani, Ryosuke

AU - Mochizuki, Kentaro

AU - Temma, Kenta

AU - Kumamoto, Yasuaki

AU - Tanaka, Hideo

AU - Fujita, Katsumasa

N1 - Funding Information: This work was supported by JST CREST and COI-NEXT Grant No. JPMJCR15N3 and JPMJPF2009, Japan. The authors thank T. Kubo of Osaka University for his valuable advice. Funding Information: This work was supported by JST CREST and COI‐NEXT Grant No. JPMJCR15N3 and JPMJPF2009, Japan. The authors thank T. Kubo of Osaka University for his valuable advice. Publisher Copyright: © 2021 Royal Microscopical Society.

PY - 2022/11

Y1 - 2022/11

N2 - We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.

AB - We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.

UR - http://www.scopus.com/inward/record.url?scp=85107755611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85107755611&partnerID=8YFLogxK

U2 - 10.1111/jmi.13033

DO - 10.1111/jmi.13033

M3 - Article

C2 - 34028848

AN - SCOPUS:85107755611

SN - 0022-2720

VL - 288

SP - 117

EP - 129

JO - Journal of Microscopy

JF - Journal of Microscopy

IS - 2

ER -

Using saturated absorption for superresolution laser scanning transmission microscopy

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this