Using saturated absorption for superresolution laser scanning transmission microscopy

Kentaro Nishida, Hikaru Sato, Ryosuke Oketani, Kentaro Mochizuki, Kenta Temma, Yasuaki Kumamoto, Hideo Tanaka, Katsumasa Fujita

Research output: Contribution to journalArticlepeer-review


We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.

Original languageEnglish
Pages (from-to)117-129
Number of pages13
JournalJournal of Microscopy
Issue number2
Publication statusPublished - Nov 2022
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology


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