Use of 13C-labeled glucose for measuring exogenous glucose oxidation in mice

Kengo Ishihara; Shinichi Oyaizu; Wataru Mizunoya; Yoshiko Fukuchi; Kyoden Yasumoto; Tohru Fushiki

doi:10.1271/bbb.66.426

Use of ¹³C-labeled glucose for measuring exogenous glucose oxidation in mice

Kengo Ishihara, Shinichi Oyaizu, Wataru Mizunoya, Yoshiko Fukuchi, Kyoden Yasumoto, Tohru Fushiki

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The author modified a respiratory gas analyzer to analyze the respiratory ¹³CO₂ of 12 small laboratory animals all at once. To investigate the practical use of this system, mice were orally (OR) or intravenously (IV) given glucose solutions containing three different amounts of ¹³C-labeled glucose. Expired ¹³CO₂ derived from exogenous glucose was detected within 10 minutes after administration in OR mice, but about 30 minutes in IV mice. The height of the peak of ¹³CO₂ expiration was correlated with the administered ¹³C-glucose mass.

Original language	English
Pages (from-to)	426-429
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	66
Issue number	2
DOIs	https://doi.org/10.1271/bbb.66.426
Publication status	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.66.426

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abstract = "The author modified a respiratory gas analyzer to analyze the respiratory 13CO2 of 12 small laboratory animals all at once. To investigate the practical use of this system, mice were orally (OR) or intravenously (IV) given glucose solutions containing three different amounts of 13C-labeled glucose. Expired 13CO2 derived from exogenous glucose was detected within 10 minutes after administration in OR mice, but about 30 minutes in IV mice. The height of the peak of 13CO2 expiration was correlated with the administered 13C-glucose mass.",

author = "Kengo Ishihara and Shinichi Oyaizu and Wataru Mizunoya and Yoshiko Fukuchi and Kyoden Yasumoto and Tohru Fushiki",

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AU - Oyaizu, Shinichi

AU - Mizunoya, Wataru

AU - Fukuchi, Yoshiko

AU - Yasumoto, Kyoden

AU - Fushiki, Tohru

PY - 2002

Y1 - 2002

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AB - The author modified a respiratory gas analyzer to analyze the respiratory 13CO2 of 12 small laboratory animals all at once. To investigate the practical use of this system, mice were orally (OR) or intravenously (IV) given glucose solutions containing three different amounts of 13C-labeled glucose. Expired 13CO2 derived from exogenous glucose was detected within 10 minutes after administration in OR mice, but about 30 minutes in IV mice. The height of the peak of 13CO2 expiration was correlated with the administered 13C-glucose mass.

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Use of ¹³C-labeled glucose for measuring exogenous glucose oxidation in mice

Abstract

All Science Journal Classification (ASJC) codes

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