Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide

Hiroya Ishikawa; Mitsuya Shimoda; Akiyoshi Yonekura; Keiko Mishima; Kiyoshi Matsumoto; Yutaka Osajima

doi:10.1271/bbb.64.1295

Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide

Hiroya Ishikawa, Mitsuya Shimoda, Akiyoshi Yonekura, Keiko Mishima, Kiyoshi Matsumoto, Yutaka Osajima

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO₂ were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO₂. No important changes were observed in heating, autoclaving, or pH-lowering.

Original language	English
Pages (from-to)	1295-1297
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	64
Issue number	6
DOIs	https://doi.org/10.1271/bbb.64.1295
Publication status	Published - 2000

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.",

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AU - Ishikawa, Hiroya

AU - Shimoda, Mitsuya

AU - Yonekura, Akiyoshi

AU - Mishima, Keiko

AU - Matsumoto, Kiyoshi

AU - Osajima, Yutaka

PY - 2000

Y1 - 2000

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AB - The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.

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IS - 6

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Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide

Abstract

All Science Journal Classification (ASJC) codes

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