Ultra-multiplex CARS spectroscopic imaging with 1-millisecond pixel dwell time

Hideaki Kano; Takumi Maruyama; Junko Kano; Yuki Oka; Daiki Kaneta; Tiffany Guerenne; Philippe Leproux; Vincent Couderc; Masayuki Noguchi

doi:10.1364/OSAC.2.001693

Ultra-multiplex CARS spectroscopic imaging with 1-millisecond pixel dwell time

Hideaki Kano, Takumi Maruyama, Junko Kano, Yuki Oka, Daiki Kaneta, Tiffany Guerenne, Philippe Leproux, Vincent Couderc, Masayuki Noguchi

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

We performed ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging by using a CCD camera with a fast readout time (<1 ms). The ultra-multiplex CARS signal of a polystyrene bead was detected in the range 600-3600 cm^-1 with resolution <10 cm^-1. The pixel dwell time was approximately 1 ms, which was limited by the readout time of the CCD camera rather than the exposure time. CARS images of 161 × 161 pixels were obtained of the polymer beads with a total data-acquisition time of approximately 28 s even with the use of a cost-effective microchip laser source. Label-free and ultra-multiplex (18 colors) imaging of living cells was also performed with an effective exposure time of 1.8 ms based on the molecular fingerprint as well as the C-H and O-H stretching vibrational modes using a master oscillator fiber amplifier laser source.

Original language	English
Pages (from-to)	1693-1705
Number of pages	13
Journal	OSA Continuum
Volume	2
Issue number	5
DOIs	https://doi.org/10.1364/OSAC.2.001693
Publication status	Published - May 15 2019
Externally published	Yes

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

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10.1364/OSAC.2.001693

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title = "Ultra-multiplex CARS spectroscopic imaging with 1-millisecond pixel dwell time",

abstract = "We performed ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging by using a CCD camera with a fast readout time (<1 ms). The ultra-multiplex CARS signal of a polystyrene bead was detected in the range 600-3600 cm-1 with resolution <10 cm-1. The pixel dwell time was approximately 1 ms, which was limited by the readout time of the CCD camera rather than the exposure time. CARS images of 161 × 161 pixels were obtained of the polymer beads with a total data-acquisition time of approximately 28 s even with the use of a cost-effective microchip laser source. Label-free and ultra-multiplex (18 colors) imaging of living cells was also performed with an effective exposure time of 1.8 ms based on the molecular fingerprint as well as the C-H and O-H stretching vibrational modes using a master oscillator fiber amplifier laser source.",

author = "Hideaki Kano and Takumi Maruyama and Junko Kano and Yuki Oka and Daiki Kaneta and Tiffany Guerenne and Philippe Leproux and Vincent Couderc and Masayuki Noguchi",

note = "Funding Information: Japan Society for the Promotion of Science (JSPS) (18H02000); Konica Minolta Imaging Science Foundation. Publisher Copyright: {\textcopyright} 2019 Optical Society of America.",

year = "2019",

month = may,

day = "15",

doi = "10.1364/OSAC.2.001693",

language = "English",

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AU - Kano, Hideaki

AU - Maruyama, Takumi

AU - Kano, Junko

AU - Oka, Yuki

AU - Kaneta, Daiki

AU - Guerenne, Tiffany

AU - Leproux, Philippe

AU - Couderc, Vincent

AU - Noguchi, Masayuki

PY - 2019/5/15

Y1 - 2019/5/15

N2 - We performed ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging by using a CCD camera with a fast readout time (<1 ms). The ultra-multiplex CARS signal of a polystyrene bead was detected in the range 600-3600 cm-1 with resolution <10 cm-1. The pixel dwell time was approximately 1 ms, which was limited by the readout time of the CCD camera rather than the exposure time. CARS images of 161 × 161 pixels were obtained of the polymer beads with a total data-acquisition time of approximately 28 s even with the use of a cost-effective microchip laser source. Label-free and ultra-multiplex (18 colors) imaging of living cells was also performed with an effective exposure time of 1.8 ms based on the molecular fingerprint as well as the C-H and O-H stretching vibrational modes using a master oscillator fiber amplifier laser source.

AB - We performed ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging by using a CCD camera with a fast readout time (<1 ms). The ultra-multiplex CARS signal of a polystyrene bead was detected in the range 600-3600 cm-1 with resolution <10 cm-1. The pixel dwell time was approximately 1 ms, which was limited by the readout time of the CCD camera rather than the exposure time. CARS images of 161 × 161 pixels were obtained of the polymer beads with a total data-acquisition time of approximately 28 s even with the use of a cost-effective microchip laser source. Label-free and ultra-multiplex (18 colors) imaging of living cells was also performed with an effective exposure time of 1.8 ms based on the molecular fingerprint as well as the C-H and O-H stretching vibrational modes using a master oscillator fiber amplifier laser source.

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JO - OSA Continuum

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ER -

Ultra-multiplex CARS spectroscopic imaging with 1-millisecond pixel dwell time

Abstract

All Science Journal Classification (ASJC) codes

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