Tunneling nanotube formation is essential for the regulation of osteoclastogenesis

Akira Takahashi, Akiko Kukita, Yin Ji Li, Jing Qi Zhang, Hisayuki Nomiyama, Takayoshi Yamaza, Yasunori Ayukawa, Kiyoshi Koyano, Toshio Kukita

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


Osteoclasts are the multinucleated giant cells formed by cell fusion of mononuclear osteoclast precursors. Despite the finding of several membrane proteins involving DC-STAMP as regulatory proteins required for fusion among osteoclast precursors, cellular and molecular events concerning this process are still ambiguous. Here we identified Tunneling Nanotubes (TNTs), long intercellular bridges with small diameters, as the essential cellular structure for intercellular communication among osteoclast precursors in prior to cell fusion. Formation of TNTs was highly associated with osteoclastogenesis and it was accompanied with the significant induction of the M-Sec gene, an essential gene for TNT formation. M-Sec gene expression was significantly upregulated by RANKL-treatment in osteoclast precursor cell line. Blockage of TNT formation by Latrunclin B or by M-Sec siRNA significantly suppressed osteoclastogenesis. We have detected the rapid intercellular transport of not only the membrane phospholipids labeled with DiI but also the DC-STAMP-GFP fusion protein through TNTs formed among osteoclast precursors during osteoclastogenesis. Transportation of such regulatory molecules through TNTs would be essential for the process of the specific cell fusion among osteoclast precursors. J. Cell. Biochem. 114: 1238-1247, 2013. © 2012 Wiley Periodicals, Inc.

Original languageEnglish
Pages (from-to)1238-1247
Number of pages10
JournalJournal of Cellular Biochemistry
Issue number6
Publication statusPublished - Jun 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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