TY - JOUR
T1 - Tsukushi is involved in the wound healing by regulating the expression of cytokines and growth factors
AU - Niimori, Daisuke
AU - Kawano, Rie
AU - Niimori-Kita, Kanako
AU - Ihn, Hironobu
AU - Ohta, Kunimasa
N1 - Funding Information:
We thank Prof. Tanaka for his valuable comments, Mrs. Iimori for her technical assistance, and all members of our lab for their valuable help. This study was supported by KAKENHI Grant Numbers 21659268 & 25860957 and the Kumamoto University Global COE Program (Cell Fate Regulation Research and Education Unit).
Publisher Copyright:
© 2014 The International CCN Society.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - During the wound-healing process, macrophages, fibroblasts, and myofibroblasts play a leading role in shifting from the inflammation phase to the proliferation phase, although little is known about the cell differentiation and molecular control mechanisms underlying these processes. Previously, we reported that Tsukushi (TSK), a member of the small leucine-rich repeat proteoglycan family, functions as a key extracellular coordinator of multiple signalling networks. In this study, we investigated the contribution of TSK to wound healing. Analysis of wound tissue in heterozygous TSK-lacZ knock-in mice revealed a pattern of sequential TSK expression from macrophages to myofibroblasts. Quantitative PCR and in vitro cell induction experiments showed that TSK controls macrophage function and myofibroblast differentiation by inhibiting TGF-β1 secreted from macrophages. Our results suggest TSK facilitates wound healing by maintaining inflammatory cell quiescence.
AB - During the wound-healing process, macrophages, fibroblasts, and myofibroblasts play a leading role in shifting from the inflammation phase to the proliferation phase, although little is known about the cell differentiation and molecular control mechanisms underlying these processes. Previously, we reported that Tsukushi (TSK), a member of the small leucine-rich repeat proteoglycan family, functions as a key extracellular coordinator of multiple signalling networks. In this study, we investigated the contribution of TSK to wound healing. Analysis of wound tissue in heterozygous TSK-lacZ knock-in mice revealed a pattern of sequential TSK expression from macrophages to myofibroblasts. Quantitative PCR and in vitro cell induction experiments showed that TSK controls macrophage function and myofibroblast differentiation by inhibiting TGF-β1 secreted from macrophages. Our results suggest TSK facilitates wound healing by maintaining inflammatory cell quiescence.
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U2 - 10.1007/s12079-014-0241-y
DO - 10.1007/s12079-014-0241-y
M3 - Article
AN - SCOPUS:85027952000
SN - 1873-9601
VL - 8
SP - 173
EP - 177
JO - Journal of Cell Communication and Signaling
JF - Journal of Cell Communication and Signaling
IS - 3
ER -