TY - JOUR
T1 - Trifluoroleucine-resistant mutant of Saccharomyces cerevisiae also exhibits pleiotropic drug resistance
AU - Oba, Takahiro
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
PY - 2008
Y1 - 2008
N2 - In this study, we revealed that a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae also exhibits pleiotropic drug resistance. DNA microarray analysis of wild-type strain vs. a trifluoroleucine-resistant mutant showed that the PDR5, PDR10, PDR15, and YOR1 genes, encoding the ABC transporter, had high levels of expression. By contrast, the 9 transcriptional factor genes of the ABC transporter, such as PDR1 and PDR3, and the repressor of drug resistance, RDR1, were not up-regulated. When the nucleotide sequences of PDR1 and PDR3 of the wild-type strain and trifluoroleucine-resistant mutant were compared, the sequences of both genes in each strain were identical.
AB - In this study, we revealed that a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae also exhibits pleiotropic drug resistance. DNA microarray analysis of wild-type strain vs. a trifluoroleucine-resistant mutant showed that the PDR5, PDR10, PDR15, and YOR1 genes, encoding the ABC transporter, had high levels of expression. By contrast, the 9 transcriptional factor genes of the ABC transporter, such as PDR1 and PDR3, and the repressor of drug resistance, RDR1, were not up-regulated. When the nucleotide sequences of PDR1 and PDR3 of the wild-type strain and trifluoroleucine-resistant mutant were compared, the sequences of both genes in each strain were identical.
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U2 - 10.1007/BF03175332
DO - 10.1007/BF03175332
M3 - Article
AN - SCOPUS:47849090186
SN - 1590-4261
VL - 58
SP - 293
EP - 296
JO - Annals of Microbiology
JF - Annals of Microbiology
IS - 2
ER -