Trehalose protects urea-induced unfolding of α-chymotrypsin

Awanish Kumar, Pankaj Attri, Pannuru Venkatesu

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Trehalose, a naturally occurring osmolyte, appears to be one of the most effective protectants for enzymes under various stress conditions while urea, a classical denaturant, destabilizes the activity, function, and alters the native structure of proteins. Herein, we have characterized the counteracting effects of trehalose on the deleterious effect of urea on α-chymotrypsin (CT) through the calorimetric data (transition temperature (Tm), enthalpy change (ΔH), heat capacity change (ΔCp) and Gibbs free energy of unfolding (ΔGu) by using differential scanning calorimeter (DSC) and circular dichroism (CD) techniques, respectively, at a 1:2 ratio of trehalose and urea, as well as various urea concentration (up to 6M) in the presence of 1M trehalose. Our parallel experimental results explicitly elucidate that trehalose strongly offset the deleterious actions of urea on CT at 1:2 molar ratio of trehalose and urea, however, trehalose (1M) some how failed to counteract the perturbation effects of urea (3-6M) on CT.

Original languageEnglish
Pages (from-to)540-545
Number of pages6
JournalInternational Journal of Biological Macromolecules
Issue number4
Publication statusPublished - Nov 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Structural Biology
  • Biochemistry


Dive into the research topics of 'Trehalose protects urea-induced unfolding of α-chymotrypsin'. Together they form a unique fingerprint.

Cite this