TY - JOUR
T1 - Treatment of poplar callus with ferulic and sinapic acids I
T2 - Incorporation and enhancement of lignin biosynthesis
AU - Hamada, Katsuyoshi
AU - Tsutsumi, Yuji
AU - Yamauchi, Kazuchika
AU - Fukushima, Kazuhiko
AU - Nishida, Tomoaki
N1 - Funding Information:
Acknowledgment This study was supported in part by a grant-in-aid from the Scientific Research Fund (10760106) of the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2003
Y1 - 2003
N2 - Ferulic acid (FA), tetradeuteroferulic acid (DFA), sinapic acid (SA), or heptadeuterosinapic acid (DSA) was exogenously supplied to poplar (Populus alba L.) callus. Administration of FA or SA increased the lignin content of the callus to about twice that of the control callus. Gas chromatographic analysis of the alkali hydrolysate of the cell wall residue revealed that only a trace amount of SA was bound to the cell wall, and the amount of FA was less than 2% of the total callus lignin. Thioacidolysis of the DFA-treated callus indicated that DFA is effectively converted to both coniferyl and sinapyl alcohols and then incorporated into the corresponding lignin. Incorporation of DSA into syringyl lignin or guaiacyl lignin was not observed, but yields of syringyl lignin thioacidolysis products were markedly increased by DSA treatment of the callus. These results suggest that SA may not be a precursor of sinapyl alcohol and syringyl lignin per se, but it may induce or enhance the biosynthesis of syringyl lignin in poplar callus.
AB - Ferulic acid (FA), tetradeuteroferulic acid (DFA), sinapic acid (SA), or heptadeuterosinapic acid (DSA) was exogenously supplied to poplar (Populus alba L.) callus. Administration of FA or SA increased the lignin content of the callus to about twice that of the control callus. Gas chromatographic analysis of the alkali hydrolysate of the cell wall residue revealed that only a trace amount of SA was bound to the cell wall, and the amount of FA was less than 2% of the total callus lignin. Thioacidolysis of the DFA-treated callus indicated that DFA is effectively converted to both coniferyl and sinapyl alcohols and then incorporated into the corresponding lignin. Incorporation of DSA into syringyl lignin or guaiacyl lignin was not observed, but yields of syringyl lignin thioacidolysis products were markedly increased by DSA treatment of the callus. These results suggest that SA may not be a precursor of sinapyl alcohol and syringyl lignin per se, but it may induce or enhance the biosynthesis of syringyl lignin in poplar callus.
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U2 - 10.1007/s10086-002-0477-7
DO - 10.1007/s10086-002-0477-7
M3 - Article
AN - SCOPUS:0142134965
SN - 1435-0211
VL - 49
SP - 333
EP - 338
JO - Journal of Wood Science
JF - Journal of Wood Science
IS - 4
ER -