Transglycosylation activity of endo-β-N- acetylglucosaminidase from Arthrobacter protophormiae

K. Takegawa, S. Yamaguchi, A. Kondo, H. Iwamoto, M. Nakoshi, I. Kato, S. Iwahara

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57 Citations (Scopus)


Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae has transglycosylation activity. Treatment of (Man)6(GlcNAc)2Asn with the enzyme in the presence of free N-acetylglucosamine gave a mixture of (Man)6GlcNAc and (Man)6GlcNAcβ1→4GlcNAc mixture. N-Acetylglucosamine at the reducing end of the latter sugar chain was found by HPLC of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 MHz 1H-NMR spectroscopy.

Original languageEnglish
Pages (from-to)849-855
Number of pages7
JournalBiochemistry International
Issue number5
Publication statusPublished - 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry


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