Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex

Katsuhide Miyake; Mikio Takahashi; Takeaki Dohda; Hidenori Kaneoka; Yoshitaka Sato; Yujin Inayoshi; Masamichi Kamihira; Shinji Iijima

doi:10.1007/s10616-006-6702-2

Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex

Katsuhide Miyake, Mikio Takahashi, Takeaki Dohda, Hidenori Kaneoka, Yoshitaka Sato, Yujin Inayoshi, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

α-Fetoprotein (AFP) is a fetal serum protein abundant in fetal liver whose expression is downregulated in adult liver. The promoter-proximal region of the AFP gene contains two binding sites for CCAAT/enhancer-binding protein α (C/EBPα), two for hepatocyte nuclear factor-1α (HNF-1α) and one for nuclear factor-1 (NF-1). Luciferase reporter assays showed that a combination of C/EBPα, HNF-1α, NF-1 and coactivator p300 gave the maximal activity but the BRG1 and BRM, ATPase subunit of the chromatin remodeling complex SWI/SNF, repressed the transactivation of the AFP gene by these transcription factors. Deletion analyses of C/EBPα binding sites suggested that C/EBPα recruited SWI/SNF complex and caused the repression. This repression may also play important roles in the downregulation of the AFP gene in adult hepatocytes.

Original language	English
Pages (from-to)	143-151
Number of pages	9
Journal	Cytotechnology
Volume	49
Issue number	2-3
DOIs	https://doi.org/10.1007/s10616-006-6702-2
Publication status	Published - Oct 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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title = "Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex",

abstract = "α-Fetoprotein (AFP) is a fetal serum protein abundant in fetal liver whose expression is downregulated in adult liver. The promoter-proximal region of the AFP gene contains two binding sites for CCAAT/enhancer-binding protein α (C/EBPα), two for hepatocyte nuclear factor-1α (HNF-1α) and one for nuclear factor-1 (NF-1). Luciferase reporter assays showed that a combination of C/EBPα, HNF-1α, NF-1 and coactivator p300 gave the maximal activity but the BRG1 and BRM, ATPase subunit of the chromatin remodeling complex SWI/SNF, repressed the transactivation of the AFP gene by these transcription factors. Deletion analyses of C/EBPα binding sites suggested that C/EBPα recruited SWI/SNF complex and caused the repression. This repression may also play important roles in the downregulation of the AFP gene in adult hepatocytes.",

author = "Katsuhide Miyake and Mikio Takahashi and Takeaki Dohda and Hidenori Kaneoka and Yoshitaka Sato and Yujin Inayoshi and Masamichi Kamihira and Shinji Iijima",

year = "2005",

month = oct,

doi = "10.1007/s10616-006-6702-2",

language = "English",

volume = "49",

pages = "143--151",

journal = "Cytotechnology",

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T1 - Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex

AU - Miyake, Katsuhide

AU - Takahashi, Mikio

AU - Dohda, Takeaki

AU - Kaneoka, Hidenori

AU - Sato, Yoshitaka

AU - Inayoshi, Yujin

AU - Kamihira, Masamichi

AU - Iijima, Shinji

PY - 2005/10

Y1 - 2005/10

N2 - α-Fetoprotein (AFP) is a fetal serum protein abundant in fetal liver whose expression is downregulated in adult liver. The promoter-proximal region of the AFP gene contains two binding sites for CCAAT/enhancer-binding protein α (C/EBPα), two for hepatocyte nuclear factor-1α (HNF-1α) and one for nuclear factor-1 (NF-1). Luciferase reporter assays showed that a combination of C/EBPα, HNF-1α, NF-1 and coactivator p300 gave the maximal activity but the BRG1 and BRM, ATPase subunit of the chromatin remodeling complex SWI/SNF, repressed the transactivation of the AFP gene by these transcription factors. Deletion analyses of C/EBPα binding sites suggested that C/EBPα recruited SWI/SNF complex and caused the repression. This repression may also play important roles in the downregulation of the AFP gene in adult hepatocytes.

AB - α-Fetoprotein (AFP) is a fetal serum protein abundant in fetal liver whose expression is downregulated in adult liver. The promoter-proximal region of the AFP gene contains two binding sites for CCAAT/enhancer-binding protein α (C/EBPα), two for hepatocyte nuclear factor-1α (HNF-1α) and one for nuclear factor-1 (NF-1). Luciferase reporter assays showed that a combination of C/EBPα, HNF-1α, NF-1 and coactivator p300 gave the maximal activity but the BRG1 and BRM, ATPase subunit of the chromatin remodeling complex SWI/SNF, repressed the transactivation of the AFP gene by these transcription factors. Deletion analyses of C/EBPα binding sites suggested that C/EBPα recruited SWI/SNF complex and caused the repression. This repression may also play important roles in the downregulation of the AFP gene in adult hepatocytes.

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JO - Cytotechnology

JF - Cytotechnology

IS - 2-3

ER -

Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex

Abstract

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