Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells

Maged F. Serag; Noritada Kaji; Claire Gaillard; Yukihiro Okamoto; Kazuyoshi Terasaka; Mohammad Jabasini; Manabu Tokeshi; Hajime Mizukami; Alberto Bianco; Yoshinobu Baba

doi:10.1021/nn102344t

Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells

Maged F. Serag, Noritada Kaji, Claire Gaillard, Yukihiro Okamoto, Kazuyoshi Terasaka, Mohammad Jabasini, Manabu Tokeshi, Hajime Mizukami, Alberto Bianco, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

211 Citations (Scopus)

Abstract

Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (<100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.

Original language	English
Pages (from-to)	493-499
Number of pages	7
Journal	ACS nano
Volume	5
Issue number	1
DOIs	https://doi.org/10.1021/nn102344t
Publication status	Published - Jan 25 2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

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10.1021/nn102344t

Cite this

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abstract = "Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (<100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.",

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AU - Serag, Maged F.

AU - Kaji, Noritada

AU - Gaillard, Claire

AU - Okamoto, Yukihiro

AU - Terasaka, Kazuyoshi

AU - Jabasini, Mohammad

AU - Tokeshi, Manabu

AU - Mizukami, Hajime

AU - Bianco, Alberto

AU - Baba, Yoshinobu

PY - 2011/1/25

Y1 - 2011/1/25

N2 - Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (<100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.

AB - Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (<100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.

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Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells

Abstract

All Science Journal Classification (ASJC) codes

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