TY - JOUR
T1 - Toxicological analysis of chlorhexidine in human serum using HPLC on a polymer-coated ODS column
AU - Kudo, Keiko
AU - Ikeda, Noriaki
AU - Kiyoshima, Akiko
AU - Hino, Yukiko
AU - Nishida, Naoki
AU - Inoue, Naohide
N1 - Funding Information:
We thank M. Ohara for language assistance. Part of this work was presented at the 19th annual meeting of the Japanese Association of Forensic Toxicology held in Hamamatsu, June 2000. A part of this work was supported by a Grant-in-Aid for Scientific Research (No. 13670427) from Ministry of Education, Science, Sports, and Culture.
PY - 2002/3
Y1 - 2002/3
N2 - A simple and reliable high-performance liquid chromatographic (HPLC) method for analyzing chlorhexidine in human serum was developed. After the addition of an internal standard, levomepromazine, 0.2 mL serum was deproteinized with 10% perchloric acid. The acidic supernatant was neutralized with 1M potassium carbonate solution, and the insoluble salt was removed by centrifugation. An aliquot of the supernatant was applied to HPLC with UV detection (260 nm). HPLC separation was achieved on a polymer-coated ODS column equilibrated with acetonitrile/water containing 0.05 % trifluoroacetic acid, 0.05% heptafluorobutyric acid, and 0.1% triethylamine (40:60, v/v). The calibration curve was linear in the concentration range from 0.05 to 50.0 μg/mL, and the lower limit of detection was 0.05 μg/mL. The accuracy and precision of the method were evaluated at concentrations of 0.5 μg/mL and 5.0 μg/mL. The coefficients of variation ranged from 4.0 to 4.5%. The concentration of chlorhexidine in the serum of a patient who died after a suspected intravenous injection of chlorhexidine gluconate was determined.
AB - A simple and reliable high-performance liquid chromatographic (HPLC) method for analyzing chlorhexidine in human serum was developed. After the addition of an internal standard, levomepromazine, 0.2 mL serum was deproteinized with 10% perchloric acid. The acidic supernatant was neutralized with 1M potassium carbonate solution, and the insoluble salt was removed by centrifugation. An aliquot of the supernatant was applied to HPLC with UV detection (260 nm). HPLC separation was achieved on a polymer-coated ODS column equilibrated with acetonitrile/water containing 0.05 % trifluoroacetic acid, 0.05% heptafluorobutyric acid, and 0.1% triethylamine (40:60, v/v). The calibration curve was linear in the concentration range from 0.05 to 50.0 μg/mL, and the lower limit of detection was 0.05 μg/mL. The accuracy and precision of the method were evaluated at concentrations of 0.5 μg/mL and 5.0 μg/mL. The coefficients of variation ranged from 4.0 to 4.5%. The concentration of chlorhexidine in the serum of a patient who died after a suspected intravenous injection of chlorhexidine gluconate was determined.
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U2 - 10.1093/jat/26.2.119
DO - 10.1093/jat/26.2.119
M3 - Article
C2 - 11916014
AN - SCOPUS:0036192208
SN - 0146-4760
VL - 26
SP - 119
EP - 122
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
IS - 2
ER -