Titration of the Alternative Complement Pathway Activity of Representative Cultured Fishes

Tomoki Yano, Yukihiro Hatayama, Hiroko Matsuyama, Miki Nakao

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48 Citations (Scopus)


Methods for assaying the alternative complement pathway activity (ACH50) of rainbow trout Salmo gairdneri, ayu Plecoglossus altivelis, carp Cyprinus carpio, tilapia Tilapia nilotica, eel Anguilla japonica, yellowtail Seriola quinqueradiata, porgy Pagrus major and flounder Paralichthys olivaceus sera were investigated. The ACH50 values of the sera of all fish except eel could be measured by incubating each serum with rabbit red blood cells (target cells) at 20°C in the presence of 10 mM EGTA and 10 mM MgCl2. The pH values and incubation periods employed were: rainbow trout, pH 7.0, 1.5 h; ayu, pH 7.0, 1.5 h; carp, pH 7.5, 1.5 h; tilapia, pH 7.0, 1.5; yellowtail, pH 7.0, 2.5 h; porgy, pH 7.5, 2.0 h; flounder, pH 7.5, 2.0 h. The hemolytic activity of eel serum was not reduced even in the presence of 10 mM EDTA, suggesting the participation of unknown factor(s) in the hemolysis. Therefore, titration of the ACH50 value of eel serum was not carried out. ACH50 values (unit/ml, mean±SD, n = 3 to 24) of the above 7 species were as follows: rainbow trout, 456±88; ayu, 880±376; carp, 51 ±14; tilapia, 684±560; yellowtail, 1016±56; porgy, 328 ±188; flounder, 508 ±60. These values are 5 to 60 times higher than those reported for mammals, suggesting that the role of the alternative pathway is much more important in fish than in mammals.

Original languageEnglish
Pages (from-to)1049-1054
Number of pages6
JournalNippon Suisan Gakkaishi (Japanese Edition)
Issue number6
Publication statusPublished - 1988

All Science Journal Classification (ASJC) codes

  • Aquatic Science


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