Thyrotropin decreases leptin production in rat adipocytes

Mitsuyo Shintani, Haruo Nishimura, Takashi Akamizu, Shin Yonemitsu, Hiroaki Masuzaki, Yoshihiro Ogawa, Kiminori Hosoda, Gen Inoue, Yasunao Yoshimasa, Kazuwa Nakao

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Leptin, which is secreted from adipocytes, has a role in the regulation of appetite and energy expenditure. The thyrotropin receptor (TSH-R) was recently found in adipocytes. We examined the effects of TSH on leptin production and lipolysis in rat epididymal adipocytes. TSH decreased the concentration of leptin in the medium time (~24 hours), and dose (~10-7 mol/L)-dependently (half-maximal inhibition [IC50] ≃ 10-9 mol/L). TSH also decreased the ob mRNA level approximately 55% in adipocytes. We confirmed the presence of TSH-R mRNA in the adipocytes by reverse transcription-polymerase chain reaction (RT-PCR). TSH stimulated glycerol release dose-dependently (IC50 ≃ 10-8 mol/L) in adipocytes. This TSH- induced glycerol release was further enhanced by adenosine deaminase (ADA). In summary, TSH reduced leptin production and stimulated lipolysis in rat epididymal adipocytes. Although the pathophysiological relevance of the regulation of leptin production and lipolysis by TSH is unknown, we speculate that TSH may affect the regulation of appetite and energy expenditure in pathophysiological states.

Original languageEnglish
Pages (from-to)1570-1574
Number of pages5
JournalMetabolism: Clinical and Experimental
Issue number12
Publication statusPublished - 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology


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