Three-dimensional phase-contrast imaging of single floating cells

Hiroaki Kobayashi; Ichirou Ishimaru; Toshiki Yasokawa; Katsumi Ishizaki; Shigeki Kuriyama; Tsutomu Masaki; Seiji Nakai; Kaoru Takegawa; Naotaka Tanaka

doi:10.1063/1.2402229

Three-dimensional phase-contrast imaging of single floating cells

Hiroaki Kobayashi, Ichirou Ishimaru, Toshiki Yasokawa, Katsumi Ishizaki, Shigeki Kuriyama, Tsutomu Masaki, Seiji Nakai, Kaoru Takegawa, Naotaka Tanaka

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A three-dimensional phase-contrast imaging technique that does not involve fluorescent labeling has been developed for observing floating cells. In this method, a single floating cell is made to rotate and images are acquired at several orientations of the cell using a phase-contrast microscope. From these two-dimensional phase-contrast images, three-dimensional cross-sectional images are obtained using the conventional computed tomography algorithm. This proposed method enabled successful rotation of a floating cell (a breast cancer cell line) and reconstruction of three-dimensional phase-contrast images. In these reconstructed three-dimensional images, the distribution of cell organelles is obtained and the cell nucleus is clearly distinguishable.

Original language	English
Article number	241117
Journal	Applied Physics Letters
Volume	89
Issue number	24
DOIs	https://doi.org/10.1063/1.2402229
Publication status	Published - 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/1.2402229

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title = "Three-dimensional phase-contrast imaging of single floating cells",

abstract = "A three-dimensional phase-contrast imaging technique that does not involve fluorescent labeling has been developed for observing floating cells. In this method, a single floating cell is made to rotate and images are acquired at several orientations of the cell using a phase-contrast microscope. From these two-dimensional phase-contrast images, three-dimensional cross-sectional images are obtained using the conventional computed tomography algorithm. This proposed method enabled successful rotation of a floating cell (a breast cancer cell line) and reconstruction of three-dimensional phase-contrast images. In these reconstructed three-dimensional images, the distribution of cell organelles is obtained and the cell nucleus is clearly distinguishable.",

author = "Hiroaki Kobayashi and Ichirou Ishimaru and Toshiki Yasokawa and Katsumi Ishizaki and Shigeki Kuriyama and Tsutomu Masaki and Seiji Nakai and Kaoru Takegawa and Naotaka Tanaka",

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year = "2006",

doi = "10.1063/1.2402229",

language = "English",

volume = "89",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics Publising LLC",

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TY - JOUR

T1 - Three-dimensional phase-contrast imaging of single floating cells

AU - Kobayashi, Hiroaki

AU - Ishimaru, Ichirou

AU - Yasokawa, Toshiki

AU - Ishizaki, Katsumi

AU - Kuriyama, Shigeki

AU - Masaki, Tsutomu

AU - Nakai, Seiji

AU - Takegawa, Kaoru

AU - Tanaka, Naotaka

N1 - Funding Information: This study was supported by Industrial Technology Research Grant Program in ‘04 from New Energy and Industrial Technology Development Organization (NEDO) of Japan.

PY - 2006

Y1 - 2006

N2 - A three-dimensional phase-contrast imaging technique that does not involve fluorescent labeling has been developed for observing floating cells. In this method, a single floating cell is made to rotate and images are acquired at several orientations of the cell using a phase-contrast microscope. From these two-dimensional phase-contrast images, three-dimensional cross-sectional images are obtained using the conventional computed tomography algorithm. This proposed method enabled successful rotation of a floating cell (a breast cancer cell line) and reconstruction of three-dimensional phase-contrast images. In these reconstructed three-dimensional images, the distribution of cell organelles is obtained and the cell nucleus is clearly distinguishable.

AB - A three-dimensional phase-contrast imaging technique that does not involve fluorescent labeling has been developed for observing floating cells. In this method, a single floating cell is made to rotate and images are acquired at several orientations of the cell using a phase-contrast microscope. From these two-dimensional phase-contrast images, three-dimensional cross-sectional images are obtained using the conventional computed tomography algorithm. This proposed method enabled successful rotation of a floating cell (a breast cancer cell line) and reconstruction of three-dimensional phase-contrast images. In these reconstructed three-dimensional images, the distribution of cell organelles is obtained and the cell nucleus is clearly distinguishable.

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JO - Applied Physics Letters

JF - Applied Physics Letters

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M1 - 241117

ER -

Three-dimensional phase-contrast imaging of single floating cells

Abstract

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