Three-dimensional imaging of intramural perineural invasion in colorectal cancer: Three-dimensional reconstruction approach with multiple immunohistochemically stained sections

Yu Miyashita, Tetsuo Ikeda, Eiji Shinto, Shinji Okano, Shotaro Korehisa, Hideyuki Shimazaki, Eiji Oki, Hideki Ueno, Yoshinao Oda, Masaki Mori

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Perineural invasion (PNI) at Auerbach's plexus in colorectal cancer (CRC), known as intramural PNI, is associated with adverse prognostic outcomes. This study aimed to characterize the three-dimensional (3D) architecture of CRC with intramural PNI and to evaluate the morphological features of tumor invasion around nerve tissue. Serial tissue sections from two cases of CRC were stained with cytokeratin AE1/AE3 and an anti-S-100 protein antibody. 3D models were reconstructed by scanning the virtual slides. In one case, intramural PNI was observed at the horizontal invasive front. The 3D reconstruction model showed tumor cells that appeared to infiltrate along the nervous meshwork, the structure of which was preserved. In the other case, intramural PNI was observed both at and behind the horizontal invasive front, and the 3D reconstruction model showed that the tumor cells appeared to be involved with nerve cells at the focal part of the horizontal invasive front. However, the nervous meshwork structure was not well identified in cancer-involved areas. This is the first study to characterize the 3D structure of tumor invasion around nerve tissue in CRC, demonstrating the morphological features of intramural PNI in CRC.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalPathology International
Volume72
Issue number5
DOIs
Publication statusPublished - May 2022

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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