Three dimensional bacteria concentration by negative dielectrophoresis

T. Hisajima, L. mao, K. Shinzato, M. Nakano, J. Suehiroa

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Thispaper reports a novel method to concentrate bacteria in three-dimension by negative dielectrophoretic (n-DEP) force in a microchannel. This was achieved by placing a thin dielectric layer on one of a pair of parallel plate electrodes. The dielectric layer having a home-plate like pentagonal shape, forms a gradient of electric field causing n-DEP. A three-dimensional numerical simulation of bacteria trajectory predicts that bacteria flowing a microchannel were three-dimensionally concentrated beneath the tip of the pentagonal dielectric thin layer. The trajectory and concentration of bacteria under n-DEP force were also experimentally confirmed using Escherichia coli cells. Bacteria moved along edges of the dielectric layer and were pushed to the opposite electrode, resulting in their concentration in three-dimension. The proposed device might be applicable to selective concentration of bacteria depending on their dielectric properties.

Original languageEnglish
Title of host publicationMaterials Science and Chemical Engineering
Number of pages6
Publication statusPublished - 2013
Event2013 International Conference on Materials Science and Chemical Engineering, MSCE 2013 - , Singapore
Duration: Feb 20 2013Feb 21 2013

Publication series

NameAdvanced Materials Research
ISSN (Print)1022-6680


Other2013 International Conference on Materials Science and Chemical Engineering, MSCE 2013

All Science Journal Classification (ASJC) codes

  • General Engineering


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