TY - JOUR
T1 - Thermosensitive Polymer Biocompatibility Based on Interfacial Structure at Biointerface
AU - Murakami, Daiki
AU - Kitahara, Yoko
AU - Kobayashi, Shingo
AU - Tanaka, Masaru
N1 - Funding Information:
This work was supported in part by Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Young Scientists B (Grant Number 15K17914) and the MEXT Project of “Integrated Research Consortium on Chemical Sciences”. This work was performed under the Cooperative Research Program of “Network Joint Research Center for Materials and Devices”. NIH3T3 cells were kindly gifted by Institute of Development, Aging and Cancer, Tohoku University (Sendai, Japan). The AFM works were carried out with the kind support of Prof. Atsushi Takahara in Kyushu University.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/5/14
Y1 - 2018/5/14
N2 - The interfacial structure of a thermosensitive biocompatible polymer, poly[2-(2-methoxyethoxy)ethyl methacrylate] (PMe2MA), at the polymer/phosphate-buffered saline (PBS) interface was investigated by atomic force microscopy. A number of nanometer scale protrusions appeared at 37 °C and disappeared at 22 °C, reversibly. This structural change occurred above the lower critical solution temperature of PMe2MA in PBS (19 °C), indicating that the formation of protrusions was explained by the microphase separation of polymer and water at the interfacial region. The protein adsorption and platelet adhesion onto PMe2MA interface were drastically restrained at 22 °C compared to that at 37 °C. Detachment of NIH3T3 cells accompanied by the dissipation of protrusions on the PMe2MA interface was also demonstrated. These results indicate that the protrusions act as the scaffold for the protein adsorption and cell adhesion.
AB - The interfacial structure of a thermosensitive biocompatible polymer, poly[2-(2-methoxyethoxy)ethyl methacrylate] (PMe2MA), at the polymer/phosphate-buffered saline (PBS) interface was investigated by atomic force microscopy. A number of nanometer scale protrusions appeared at 37 °C and disappeared at 22 °C, reversibly. This structural change occurred above the lower critical solution temperature of PMe2MA in PBS (19 °C), indicating that the formation of protrusions was explained by the microphase separation of polymer and water at the interfacial region. The protein adsorption and platelet adhesion onto PMe2MA interface were drastically restrained at 22 °C compared to that at 37 °C. Detachment of NIH3T3 cells accompanied by the dissipation of protrusions on the PMe2MA interface was also demonstrated. These results indicate that the protrusions act as the scaffold for the protein adsorption and cell adhesion.
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U2 - 10.1021/acsbiomaterials.8b00081
DO - 10.1021/acsbiomaterials.8b00081
M3 - Article
C2 - 33445316
AN - SCOPUS:85046954572
SN - 2373-9878
VL - 4
SP - 1591
EP - 1597
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 5
ER -