The stage of negative selection in tolerance induction in neonatal mice

T. Ando, Y. Yoshikai, G. Matsuzaki, H. Takimoto, K. Nomoto

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4 Citations (Scopus)


In flow microfluorometry analysis of thymus and lymph node cells of C57BL/6(I-E-,Mlsb) mice rendered neonatally tolerant to (C57BL/6 x AKR/J)F1 (I-E+,Mls(b/a)) lymphoid cells, both CD4+ and CD8+ cells showed a striking reduction in the number of Vβ6+ cells capable of recognizing Mlsa in the context of major histocompatibility complex (MHC) class II molecules, indicating that clonal deletion of Vβ6+ cells by Mlsa antigen occurs just at a stage of immature Vβ6(low)CD4+CD8+ thymocytes. On the other hand, the number of Vβ11+ cells capable of recognizing I-E was markedly reduced in CD4+ cells, but CD8+ cells showed only partial (20%) reduction of such a population. The clonal deletion of Vβ11+ cells by I-E may begin at the transitional stage from Vβ11(low)CD4+CD8+ to Vβ11(high)CD4+CD8- single-position cells, and Vβ11(low)CD4+CD8+ cells differentiating to Vβ11(high)CD4-CD8+ cells seem to be resistant to clonal deletion. Vβ11+ T cells are also stimulated by staphylococcal enterotoxin A (SEA) irrespective of expression of CD4 or CD8. Nearly all of both Vβ11+CD4+ and Vβ11+CD8+ lymph node T cells were deleted by the injection of SEA every other day from birth. In their thymi, both Vβ11+CD4+CD8- and Vβ11+CD4-CD8+ single-positive thymocytes were deleted, and the proportion of Vβ11(low) thymocytes was lower than that of normal mice. The clonal deletion of Vβ11+ T cells by SEA injection occurs at a stage of immature Vβ11(low)CD4+CD8+ double-positive thymocytes, resulting in deletion of both Vβ11+CD4+ and Vβ11+CD8+ T cells.

Original languageEnglish
Pages (from-to)638-644
Number of pages7
Issue number4
Publication statusPublished - 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology


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