TY - JOUR
T1 - The Schizosaccharomyces pombe gms1+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation
AU - Tabuchi, Mitsuaki
AU - Tanaka, Naotaka
AU - Iwahara, Shojiro
AU - Takegawa, Kaoru
N1 - Funding Information:
We gratefully acknowledge the gift from Takashi Toda, Chikashi Shimoda, Koei Okazaki of the S. pombe strains and plasmids. We thank Clinton Ballou, Scott Emr, Nobuhiro Ishida, and Masao Ka-wakita for valuable discussions and unpublished information. This work was supported in part by a Grant-in-aid for Scienti®c Research from the Ministry of Education, Science, Sports and Culture of Japan (to K.T.). The gms1/ nucleotide sequence will appear in DDBJ, EMBL, and GenBank nucleotide sequence databases with the following accession number D89616.
PY - 1997/3/6
Y1 - 1997/3/6
N2 - In a previous study, we isolated a Schizosaccharomyces pombe mutant defective in protein galactosylation. From an S. pombe genomic library, we cloned the gms1+ gene which restored the galactosylation of cell wall glycoproteins. Gms1 protein shares significant sequence similarity with human UDP-galactose and murine CMP-sialic acid transporters. The fission yeast strains deleted for the gms1+ gene lacked galactose residues in sell surface glycoproteins and were significantly decreased in UDP-galactose transport activity. These results showed that the gms1+ encodes an UDP-galactose transporter, and this protein appears to be an essential role for the incorporation of UDP-galactose into the lumen of Golgi in S. pombe.
AB - In a previous study, we isolated a Schizosaccharomyces pombe mutant defective in protein galactosylation. From an S. pombe genomic library, we cloned the gms1+ gene which restored the galactosylation of cell wall glycoproteins. Gms1 protein shares significant sequence similarity with human UDP-galactose and murine CMP-sialic acid transporters. The fission yeast strains deleted for the gms1+ gene lacked galactose residues in sell surface glycoproteins and were significantly decreased in UDP-galactose transport activity. These results showed that the gms1+ encodes an UDP-galactose transporter, and this protein appears to be an essential role for the incorporation of UDP-galactose into the lumen of Golgi in S. pombe.
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U2 - 10.1006/bbrc.1997.6239
DO - 10.1006/bbrc.1997.6239
M3 - Article
C2 - 9125114
AN - SCOPUS:0031555881
SN - 0006-291X
VL - 232
SP - 121
EP - 125
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -