TY - JOUR
T1 - The ODN probes conjugating the Cu(II) complex enhance the luminol chemiluminescence by assembling on the DNA template
AU - Taniguchi, Yosuke
AU - Nitta, Akiko
AU - Park, Sun Min
AU - Kohara, Akiko
AU - Uzu, Takahiro
AU - Sasaki, Shigeki
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (S) from Japan Society for the Promotion of Science (JSPS) , CREST from Japan Science and Technology Agency . We acknowledge Dr. Kenji Yoza at BRUKER AXS for the X-ray study.
PY - 2010/12/15
Y1 - 2010/12/15
N2 - Potent peroxidase-like activity of the β-ketoenamine (1)-dicopper (II) complex (2) for the chemiluminescence (CL) of luminol either in the presence or absence of H2O2 has been previously demonstrated by our group. In this study, the β-ketoenamine (1) as the ligand unit for copper(II) was incorporated into the oligonucleotide (ODN) probes. It has been shown that the catalytic activity of the ODN probes conjugating the ligand-Cu(II) complex is activated by hybridization with the target DNA with the complementary sequence. Thus, this study has successfully demonstrated the basic concept for the sensitive detection of nucleic acids by CL based on the template-inductive activation of the catalytic unit for CL.
AB - Potent peroxidase-like activity of the β-ketoenamine (1)-dicopper (II) complex (2) for the chemiluminescence (CL) of luminol either in the presence or absence of H2O2 has been previously demonstrated by our group. In this study, the β-ketoenamine (1) as the ligand unit for copper(II) was incorporated into the oligonucleotide (ODN) probes. It has been shown that the catalytic activity of the ODN probes conjugating the ligand-Cu(II) complex is activated by hybridization with the target DNA with the complementary sequence. Thus, this study has successfully demonstrated the basic concept for the sensitive detection of nucleic acids by CL based on the template-inductive activation of the catalytic unit for CL.
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U2 - 10.1016/j.bmc.2010.10.007
DO - 10.1016/j.bmc.2010.10.007
M3 - Article
C2 - 21115284
AN - SCOPUS:78649445917
SN - 0968-0896
VL - 18
SP - 8614
EP - 8617
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
IS - 24
ER -