TY - JOUR
T1 - The occurrence of geometric polyprenol isomers in the rubber- producing plant, Eucommia ulmoides oliver
AU - Bamba, Takeshi
AU - Fukusaki, Ei Ichiro
AU - Kajivama, Shin Ichiro
AU - Ute, Koichi
AU - Kitayama, Tatsuki
AU - Kobayashi, Akio
N1 - Funding Information:
This work was supported by the New Energy and Industrial Technology Development Organization (NEDO). The authors are very grateful to Dr. A. Okazawa and Dr. Y. Nakazawa for helpful discussions and support of the investigation, to Hitachi Zosen Corporation for providing E. ulmoides leaves, and to Mr. T. Ozaki for his help in performing the chromatographic and NMR experiments. Thanks are due to the Venture Business Laboratory, Osaka University, for assistance in performing the NMR experiments on a Varian Unity-INOVA 750 spectrometer and to the Instrumental Analysis Center (Dr. H. Moriguchi), Faculty of Engineering, Osaka University, for assistance in obtaining field desorption-MS spectra on a JEOL JMS-DX303.
PY - 2001
Y1 - 2001
N2 - The chain length and geometric isomerism of polyprenols from Eucommia ulmoides Oliver were analyzed using supercritical fluid chromatography. After intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and -cis prenols. The presence of longchain poly-trans prenols (>9 mers) was confirmed for the first time in plants. Trans isomers were found in the leaf, seed coat, and root, but not in the bark and seed. Poly-trans prenols in this plant may act as intermediates for trans-polyisoprene biosynthesis.
AB - The chain length and geometric isomerism of polyprenols from Eucommia ulmoides Oliver were analyzed using supercritical fluid chromatography. After intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and -cis prenols. The presence of longchain poly-trans prenols (>9 mers) was confirmed for the first time in plants. Trans isomers were found in the leaf, seed coat, and root, but not in the bark and seed. Poly-trans prenols in this plant may act as intermediates for trans-polyisoprene biosynthesis.
UR - http://www.scopus.com/inward/record.url?scp=0034888574&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034888574&partnerID=8YFLogxK
U2 - 10.1007/s11745-001-0778-5
DO - 10.1007/s11745-001-0778-5
M3 - Article
C2 - 11521971
AN - SCOPUS:0034888574
SN - 0024-4201
VL - 36
SP - 727
EP - 732
JO - Lipids
JF - Lipids
IS - 7
ER -