The novel heme-dependent inducible protein, SRRD regulates heme biosynthesis and circadian rhythms

Yuka Adachi, Mana Umeda, Asako Kawazoe, Tetsuya Sato, Yasuyuki Ohkawa, Sakihito Kitajima, Shingo Izawa, Ikuko Sagami, Shigeru Taketani

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Heme plays a role in the regulation of the expression of genes related to circadian rhythms and heme metabolism. In order to identify new heme-regulated proteins, an RNA sequence analysis using mouse NIH3T3 cells treated without or with 5-aminolevulinic acid (ALA) was performed. Among the changes observed in the levels of various mRNAs including heme oxygenase-1 (HO-1) and ALA synthase-1 (ALAS1), a mouse homologue of the plant circadian-regulating protein SRR1, SRR1 domain containing (SRRD) was induced by the ALA treatment. The expression of SRRD was dependent on heme biosynthesis, and increased the production of heme. SRRD was expressed under circadian rhythms, and influenced the expression of clock genes including PER2, BMAL1, and CLOCK. The knockout of SRRD arrested the growth of cells, indicating that SRRD plays roles in heme-regulated circadian rhythms and cell proliferation.

    Original languageEnglish
    Pages (from-to)19-29
    Number of pages11
    JournalArchives of Biochemistry and Biophysics
    Volume631
    DOIs
    Publication statusPublished - Oct 1 2017

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology

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