TY - JOUR
T1 - The making of symbiont capsule in the plataspid stinkbug Megacopta punctatissima
AU - Hosokawa, Takahiro
AU - Kikuchi, Yoshitomo
AU - Xien, Ying Meng
AU - Fukatsu, Takema
N1 - Funding Information:
We thank, K. Nomura, H. Ohta, H. Ouchi, A. Sugimura, S. Suo, and S. Tatsuno for technical and secretarial assistance. This research was supported by the Program for Promotion of Basic Research Activities for Innovation Biosciences (ProBRAIN) of the Bio-Oriented Technology Research Advancement Institution. T.H. and Y.K. were supported by the Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2005/11
Y1 - 2005/11
N2 - In stinkbugs of the family Plataspidae, adult females deposit small brownish particles, containing specific symbiotic bacteria inside, on the underside of their egg mass. Newborn nymphs ingest the content of the unique structure, called "symbiont capsule", whereby vertical transmission of the symbiont occurs. We investigated the fine structure and the formation process of the symbiont capsule in the Japanese common plataspid stinkbug, Megacopta punctatissima, by using light and electron microscopy. It was demonstrated that (i) the capsule consists of three structural components, namely "symbionts", "matrix" and "envelope"; (ii) the posterior midgut of adult females is characterized by several specific sections with peculiar anatomical traits, including "thin crypt-bearing midgut (TCM) section", "swollen crypt-bearing midgut (SCM) section" and "brownish enlarged midgut (BEM) end section"; (iii) the different capsule components, symbionts, matrix and envelope, are produced and/or supplied by the specialized midgut sections, TCM, SCM and BEM, respectively; and (iv) the capsule components are stored in BEM and excreted during oviposition to produce the symbiont capsules. These results strongly suggested that the host insect incurs a substantial cost for the symbiont transmission. Ecological and evolutionary implications of the highly developed, female-specific system for symbiont transmission were discussed.
AB - In stinkbugs of the family Plataspidae, adult females deposit small brownish particles, containing specific symbiotic bacteria inside, on the underside of their egg mass. Newborn nymphs ingest the content of the unique structure, called "symbiont capsule", whereby vertical transmission of the symbiont occurs. We investigated the fine structure and the formation process of the symbiont capsule in the Japanese common plataspid stinkbug, Megacopta punctatissima, by using light and electron microscopy. It was demonstrated that (i) the capsule consists of three structural components, namely "symbionts", "matrix" and "envelope"; (ii) the posterior midgut of adult females is characterized by several specific sections with peculiar anatomical traits, including "thin crypt-bearing midgut (TCM) section", "swollen crypt-bearing midgut (SCM) section" and "brownish enlarged midgut (BEM) end section"; (iii) the different capsule components, symbionts, matrix and envelope, are produced and/or supplied by the specialized midgut sections, TCM, SCM and BEM, respectively; and (iv) the capsule components are stored in BEM and excreted during oviposition to produce the symbiont capsules. These results strongly suggested that the host insect incurs a substantial cost for the symbiont transmission. Ecological and evolutionary implications of the highly developed, female-specific system for symbiont transmission were discussed.
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U2 - 10.1016/j.femsec.2005.06.002
DO - 10.1016/j.femsec.2005.06.002
M3 - Article
C2 - 16332344
AN - SCOPUS:26244442246
SN - 0168-6496
VL - 54
SP - 471
EP - 477
JO - FEMS microbiology ecology
JF - FEMS microbiology ecology
IS - 3
ER -