TY - JOUR
T1 - The induction of chromosome condensation in tsBN2, a temperature-sensitive mutant of BHK21, inhibited by the calmodulin antagonist, W-7
AU - Nishimoto, Takeharu
AU - Ajiro, Kozo
AU - Hirata, Masato
AU - Yamashita, Katsumi
AU - Sekiguchi, Mutsuo
N1 - Funding Information:
This investigation was supported in part by scientific and cancer research grants from the Ministry of Education, Science and Culture of Japan, and by a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare of Japan.
PY - 1985/2
Y1 - 1985/2
N2 - The induction of premature chromosome condensation (PCC) in tsBN2 cells, a temperature-sensitive (ts) mutant of BHK21/13 which shows PCC at the non-permissive temperature, was almost completely inhibited by 40 μM W-7, an antagonist of calmodulin. The mitotic phosphorylation of histone H1 and H3 was also inhibited by W-7, W-5, a chlorine-deficient analogue of W-7 and which interacts weakly with calmodulin, did not inhibit the induction of PCC, even at a dose of 80 μM. The content of calmodulin in tsBN2 cells was increased by a temperature shift to 40.5°C. All these results suggested that calmodulin is required for the chromosome condensation.
AB - The induction of premature chromosome condensation (PCC) in tsBN2 cells, a temperature-sensitive (ts) mutant of BHK21/13 which shows PCC at the non-permissive temperature, was almost completely inhibited by 40 μM W-7, an antagonist of calmodulin. The mitotic phosphorylation of histone H1 and H3 was also inhibited by W-7, W-5, a chlorine-deficient analogue of W-7 and which interacts weakly with calmodulin, did not inhibit the induction of PCC, even at a dose of 80 μM. The content of calmodulin in tsBN2 cells was increased by a temperature shift to 40.5°C. All these results suggested that calmodulin is required for the chromosome condensation.
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U2 - 10.1016/0014-4827(85)90542-7
DO - 10.1016/0014-4827(85)90542-7
M3 - Article
C2 - 3967683
AN - SCOPUS:0021959299
SN - 0014-4827
VL - 156
SP - 351
EP - 358
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -