The GINS complex from Pyrococcus furiosus stimulates the MCM helicase activity

Takehiro Yoshimochi, Ryosuke Fujikane, Miyuki Kawanami, Fujihiko Matsunaga, Yoshizumi Ishino

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54 Citations (Scopus)


Pyrococcus furiosus, a hyperthermophilic Archaea, has homologs of the eukaryotic MCM (mini-chromosome maintenance) helicase and GINS complex. The MCM and GINS proteins are both essential factors to initiate DNA replication in eukaryotic cells. Many biochemical characterizations of the replication-related proteins have been reported, but it has not been proved that the homologs of each protein are also essential for replication in archaeal cells. Here, we demonstrated that the P. furiosus GINS complex interacts with P. furiosus MCM. A chromatin immunoprecipitation assay revealed that the GINS complex is detected preferentially at the oriC region on Pyrococcus chromosomal DNA during the exponential growth phase but not in the stationary phase. Furthermore, the GINS complex stimulates both the ATPase and DNA helicase activities of MCM in vitro. These results strongly suggest that the archaeal GINS is involved in both the initiation and elongation processes ofDNAreplication in P. furiosus, as observed in eukaryotic cells.

Original languageEnglish
Pages (from-to)1601-1609
Number of pages9
JournalJournal of Biological Chemistry
Issue number3
Publication statusPublished - Jan 18 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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