The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity

Ken Ichi Yoritsune, Tomohiko Matsuzawa, Takao Ohashi, Kaoru Takegawa

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


N-Glycan from the fission yeast Schizosaccharomyces pombe contains outer-chain pyruvic acid 4,6-ketal-linked galactose (PvGal). Here, we characterized a putative S. pombe pyruvyltransferase, Pvg1p, reported to be essential for biosynthesis of PvGal. When p-nitrophenyl-β-Gal (pNP-β-Gal) was used as a substrate, the structure of the recombinant Pvg1p product was determined to be pNP-PvGal by one- and two-dimensional NMR spectroscopy. The recombinant Pvg1p transferred pyruvyl residues from phosphoenolpyruvate specifically to β-linked galactose.

Original languageEnglish
Pages (from-to)917-921
Number of pages5
JournalFEBS Letters
Issue number7
Publication statusPublished - Apr 2 2013

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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