@article{3df6ee63df2a41dab3aa70e31cb72b4b,
title = "The expression level of pd-l1 (CD274) mrna in peripheral blood is a potential biomarker for predicting recurrence in breast cancer",
abstract = "Background/Aim: Programmed death-ligand 1 (PD-L1/CD274) elicits T-cell anergy, leading to immune suppression. We aimed to determine the prognostic relevance of PD-L1 expression in the blood of breast cancer (BC) patients. Materials and Methods: We measured PD-L1 mRNA expression in blood and tumor tissues of BC patients using RT-qPCR and a dataset from The Cancer Genome Atlas, and performed a survival analysis of PD-L1 expression in the blood of 330 BC patients. Flow cytometric analysis was performed using blood cells. Results: No statistical difference in PD-L1 expression was seen between normal controls and BC in blood or tissues. There was a significant positive correlation between the PD-L1 expression levels in blood and tissues. Decreased PD-L1 expression in blood or tissues was associated with poor recurrence-free survival. PD-L1 is mainly expressed in polymorphonuclear leukocytes. Conclusion: Low expression of PD-L1 in the blood could serve as a biomarker of poor prognosis in BC patients.",
author = "Takaaki Masuda and Miwa Noda and Akihiro Kitagawa and Qingjiang Hu and Atsushi Fujii and Shuhei Ito and Keisuke Kosai and Yuki Ando and Yoshihiro Matsumoto and Hajime Ohtsu and Hiroki Uchida and Shinji Ohno and Koshi Mimori",
note = "Funding Information: The Authors would like to thank Dr. Tyler Lahusen for the helpful comments and English proofreading. The Authors would also like to thank K. Oda, M. Kasagi, S. Sakuma, N. Mishima and T. Kawano for their excellent technical assistance. This work was supported in part by the following grants and foundations: Japan Agency for Medical Research and Development (16ck0106160h0002, 18ae0101016, 18cm0106504, 18kk0205003, and 18ck0106259); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Science Research (20K17556, 20K16416, 20H05039, 20K08930, 20K08960, 19H03715, 19K09176, 16K09220, 19K18057, 18K08649, and 18K15323); JST AIP-PRISM (JPMJCR18Y5); OITA Cancer Research Foundation; Priority Issue on Post-K computer (hp170227, hp160219, and hp170227). Funding Information: The Authors would like to thank Dr. Tyler Lahusen for the helpful comments and English proofreading. The Authors would also like to thank K. Oda, M. Kasagi, S . S akuma, N. Mishima and T. Kawano for their excellent technical assistance. This work was supported in part by the following grants and foundations: Japan Agency for Medical Research and Development (16ck0106160h0002, 18ae0101016, 18cm0106504, 18kk0205003, and 18ck0106259); Japan S ociety for the Promotion of S cience (JS PS ) Grant-in-Aid for S cience Research (20K17556, 20K16416, 20H05039, 20K08930, 20K08960, 19H03715, 19K09176, 16K09220, 19K18057, 18K08649, and 18K15323); JS T AIP-PRIS M (JPMJCR18Y5); OITA Cancer Research Foundation; Priority Issue on Post-K computer (hp170227, hp160219, and hp170227). Publisher Copyright: {\textcopyright} 2020 International Institute of Anticancer Research. All rights reserved.",
year = "2020",
month = jul,
doi = "10.21873/ANTICANRES.14362",
language = "English",
volume = "40",
pages = "3733--3742",
journal = "Anticancer research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "7",
}
