The effects of ototoxic drugs on mechano-electric transduction channels in chick cochlear hair cells

T. Kimitsuki; T. Nakagawa; K. Hisashi; S. Komune; T. Uemura

doi:10.1007/BF02565220

The effects of ototoxic drugs on mechano-electric transduction channels in chick cochlear hair cells

T. Kimitsuki, T. Nakagawa, K. Hisashi, S. Komune, T. Uemura

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Abstract

The effects of ototoxic drugs on mechano-electrical transduction (MET) currents were investigated in dissociated cochlear hair cells of the chick, using a wholecell patch-electrode voltage-clamp technique. Dihydrostreptomycin (DHSM) and cisplatin (cis-dichlorodiammine platinum II, CDDP) blocked the MET channel in a dose- and voltage-dependent manner. In contrast, acetyl salicylate did not suppress the MET current. At -50 mV, DHSM and CDDP blocked the MET channel with a Hill coefficient of 0.93 and 2.1, respectively. These findings suggest that a single DHSM molecule or more than one CDDP molecule binds to a single MET channel.

Original language	English
Pages (from-to)	S53-S56
Journal	European Archives of Oto-Rhino-Laryngology
Volume	251
Issue number	1 Supplement
DOIs	https://doi.org/10.1007/BF02565220
Publication status	Published - Aug 1994

All Science Journal Classification (ASJC) codes

Otorhinolaryngology

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10.1007/BF02565220

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abstract = "The effects of ototoxic drugs on mechano-electrical transduction (MET) currents were investigated in dissociated cochlear hair cells of the chick, using a wholecell patch-electrode voltage-clamp technique. Dihydrostreptomycin (DHSM) and cisplatin (cis-dichlorodiammine platinum II, CDDP) blocked the MET channel in a dose- and voltage-dependent manner. In contrast, acetyl salicylate did not suppress the MET current. At -50 mV, DHSM and CDDP blocked the MET channel with a Hill coefficient of 0.93 and 2.1, respectively. These findings suggest that a single DHSM molecule or more than one CDDP molecule binds to a single MET channel.",

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T1 - The effects of ototoxic drugs on mechano-electric transduction channels in chick cochlear hair cells

AU - Kimitsuki, T.

AU - Nakagawa, T.

AU - Hisashi, K.

AU - Komune, S.

AU - Uemura, T.

PY - 1994/8

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AB - The effects of ototoxic drugs on mechano-electrical transduction (MET) currents were investigated in dissociated cochlear hair cells of the chick, using a wholecell patch-electrode voltage-clamp technique. Dihydrostreptomycin (DHSM) and cisplatin (cis-dichlorodiammine platinum II, CDDP) blocked the MET channel in a dose- and voltage-dependent manner. In contrast, acetyl salicylate did not suppress the MET current. At -50 mV, DHSM and CDDP blocked the MET channel with a Hill coefficient of 0.93 and 2.1, respectively. These findings suggest that a single DHSM molecule or more than one CDDP molecule binds to a single MET channel.

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IS - 1 Supplement

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The effects of ototoxic drugs on mechano-electric transduction channels in chick cochlear hair cells

Abstract

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