TY - JOUR
T1 - The development and characterization of near-isogenic and pyramided lines carrying resistance genes to brown planthopper with the genetic background of japonica rice (Oryza sativa l.)
AU - Nguyen, Cuong D.
AU - Verdeprado, Holden
AU - Zita, Demeter
AU - Sanada-Morimura, Sachiyo
AU - Matsumura, Masaya
AU - Virk, Parminder S.
AU - Brar, Darshan S.
AU - Horgan, Finbarr G.
AU - Yasui, Hideshi
AU - Fujita, Daisuke
N1 - Funding Information:
This work was supported by JSPS KAKENHI, grant numbers JP15H04438 and 17K07606. Acknowledgments: We thank staff of the Insect Pest Management Research Group, Kyushu Okinawa Agricultural Research Center, for rearing and preparing insect populations, and Atsushi Yoshimura, Yoshiyuki Yamagata and the staff belonging to the Plant Breeding Laboratory at Kyushu University for growing and maintaining the plant materials. Furthermore, we thank Kshirod K. Jena for aiding in the development of materials and Elmer Sanchez for developing materials and taking care of the plants. We also wish to thank the Government of Vietnam for the doctoral fellowship granted to C.D.N.
Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. T.
PY - 2019/11
Y1 - 2019/11
N2 - The brown planthopper (BPH: Nilaparvata lugens Stål.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice’s resistance to BPH in the context of regionally increasing levels of virulence.
AB - The brown planthopper (BPH: Nilaparvata lugens Stål.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice’s resistance to BPH in the context of regionally increasing levels of virulence.
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U2 - 10.3390/plants8110498
DO - 10.3390/plants8110498
M3 - Article
AN - SCOPUS:85075170051
SN - 2223-7747
VL - 8
JO - Plants
JF - Plants
IS - 11
M1 - 498
ER -