The design of antimicrobial peptides based on the novel concept

Recently, we have developed a new type of antimicrobial peptides based on nuclear localization signal (NLS) sequences. We also have found that a globotriaosyl (Gb3) mimic peptide bound Shiga toxins strongly and neutralized the toxins effectively. The present paper proposes the design of tandem fusion peptides between NLS peptides and Gb3 mimic peptides. The tandem fusion peptides expressed multifunctions of both antimicrobial and Stx neutralization activities. The antimicrobial activity of the NLS peptide has been strongly enhanced by combining the Gb3 mimic sequence to its N-terminal. This proposed fusion peptides are expected to destroy bacteria and neutralize the released toxins simultaneously.