The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization

Chikako Tsutsumi, Koh Hei Sonoda, Kensuke Egashira, Hong Qiao, Toshio Hisatomi, Shintaro Nakao, Minako Ishibashi, Israel F. Charo, Taiji Sakamoto, Toshinori Murata, Tatsuro Ishibashi

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248 Citations (Scopus)


Choroidal neovascularization (CNV) is directly related to visual loss in some eye diseases, such as age-related macular degeneration. Although several human histological studies have suggested the participation of macrophages in CNV formation, the precise mechanisms are still not fully understood. In this study, we elucidated the role of ocular-infiltrating macrophages in experimental CNV using CCR2 knockout (KO) mice, wild-type mice, and C57BL/6 (B6) mice. CCR2 is the receptor of monocyte chemoattractant protein-1, and the number of infiltrating macrophage and the area of CNV were significantly reduced in CCR2 KO mice. Enriched ocular-infiltrating macrophages from B6 mice actually showed angiogenic ability in a dorsal air sac assay. Moreover, their expression of class II, CD40, B7-1 and B7-2 molecules, and the mRNA for potential angiogenic factors, such as vascular endothelial growth factor, basic fibroblast growth factor, and tumor necrosis factor α, was also observed. Collectively, we conclude that ocular-infiltrating macrophages play an important role in CNV generation.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalJournal of Leukocyte Biology
Issue number1
Publication statusPublished - Jul 2003

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Cell Biology


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