TY - JOUR
T1 - The cooperative induction of macrophage activation by fucoidan derived from Cladosiphon okamuranus and β-glucan derived from Saccharomyces cerevisiae
AU - Miyazaki, Yoshiyuki
AU - Iwaihara, Yuri
AU - Bak, Juneha
AU - Nakano, Hayato
AU - Takeuchi, S.
AU - Takeuchi, Hideaki
AU - Matsui, Toshiro
AU - Tachikawa, Daisuke
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science ( JSPS KAKENHI Grant Number 17K07819 to Y.M.). The laser scanning confocal microscope TCS SP8 used in this study was owned by Center for advanced instrumental and educational supports, Faculty of Agriculture, Kyushu University. Author contributions were as below; Y.M., and Y.I. performed all the analytical experiments. J.B. performed the analytical experiments showing in Figure 2. Y.M., Y.I., and J.B. analyzed the results. Y.M., Y.I., H.N., S.T., H.T., T.M., and D.T. considered and discussed the results and designed experimental strategy. Y.M., and Y.I. wrote the manuscript. Y.M. designed the study, edited the manuscript, and supervised the whole project. All authors have approved the final manuscript to be published.
Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (JSPS KAKENHI Grant Number 17K07819 to Y.M.). The laser scanning confocal microscope TCS SP8 used in this study was owned by Center for advanced instrumental and educational supports, Faculty of Agriculture, Kyushu University. Author contributions were as below; Y.M. and Y.I. performed all the analytical experiments. J.B. performed the analytical experiments showing in Figure 2. Y.M. Y.I. and J.B. analyzed the results. Y.M. Y.I. H.N. S.T. H.T. T.M. and D.T. considered and discussed the results and designed experimental strategy. Y.M. and Y.I. wrote the manuscript. Y.M. designed the study, edited the manuscript, and supervised the whole project. All authors have approved the final manuscript to be published.
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/8/13
Y1 - 2019/8/13
N2 - The present study investigated immune stimulatory effects of Cladosiphon okamuranus-derived fucoidan to activate murine macrophage-like cell line RAW264, and the functional relationship with zymosan, a Saccharomyces cerevisiae-derived β-glucan. The production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in RAW264 cells were remarkably enhanced in the presence of 10 μg/mL fucoidan, and the stimulatory effects of fucoidan were maximally augmented in combinational treatment with 500 ng/mL zymosan, whereas any TLR ligands had no those effects. Confocal microscopic analyses suggested that fucoidan bound on plasma membrane, and it was estimated that some cell surface molecules acted as receptor for fucoidan because cytochalasin D, an inhibitor of phagocytosis, did not affect the immune enhancing activities, whereas methyl-β-cyclodextrin (MβCD), a general agent for disruption of lipid rafts, diminished that. Furthermore, it was revealed that the additive effects of zymosan on the immune activation with fucoidan was thought to be mediated by dectin-1 based on the results with dectin-1-knockdown RAW264 cells. All of results suggested that fucoidan and some kinds of β-glucan would cooperatively reinforce the activity of innate immune cells via interactive receptor crosstalk.
AB - The present study investigated immune stimulatory effects of Cladosiphon okamuranus-derived fucoidan to activate murine macrophage-like cell line RAW264, and the functional relationship with zymosan, a Saccharomyces cerevisiae-derived β-glucan. The production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in RAW264 cells were remarkably enhanced in the presence of 10 μg/mL fucoidan, and the stimulatory effects of fucoidan were maximally augmented in combinational treatment with 500 ng/mL zymosan, whereas any TLR ligands had no those effects. Confocal microscopic analyses suggested that fucoidan bound on plasma membrane, and it was estimated that some cell surface molecules acted as receptor for fucoidan because cytochalasin D, an inhibitor of phagocytosis, did not affect the immune enhancing activities, whereas methyl-β-cyclodextrin (MβCD), a general agent for disruption of lipid rafts, diminished that. Furthermore, it was revealed that the additive effects of zymosan on the immune activation with fucoidan was thought to be mediated by dectin-1 based on the results with dectin-1-knockdown RAW264 cells. All of results suggested that fucoidan and some kinds of β-glucan would cooperatively reinforce the activity of innate immune cells via interactive receptor crosstalk.
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U2 - 10.1016/j.bbrc.2019.06.010
DO - 10.1016/j.bbrc.2019.06.010
M3 - Article
C2 - 31221482
AN - SCOPUS:85067311377
SN - 0006-291X
VL - 516
SP - 245
EP - 250
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -