The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNa from Pyrococcus horikoshii OT3

Tadashi Hara; Atsushi Terada; Hiroki Yamaguchi; Takashi Nakashima; Yoshimitsu Kakuta; Makoto Kimura

doi:10.1271/bbb.110110

The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNa from Pyrococcus horikoshii OT3

Tadashi Hara, Atsushi Terada, Hiroki Yamaguchi, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

Original language	English
Pages (from-to)	816-819
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	75
Issue number	4
DOIs	https://doi.org/10.1271/bbb.110110
Publication status	Published - 2011

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.110110

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title = "The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNa from Pyrococcus horikoshii OT3",

abstract = "We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.",

author = "Tadashi Hara and Atsushi Terada and Hiroki Yamaguchi and Takashi Nakashima and Yoshimitsu Kakuta and Makoto Kimura",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (no. 22380062 to M.K.).",

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T1 - The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNa from Pyrococcus horikoshii OT3

AU - Hara, Tadashi

AU - Terada, Atsushi

AU - Yamaguchi, Hiroki

AU - Nakashima, Takashi

AU - Kakuta, Yoshimitsu

AU - Kimura, Makoto

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (no. 22380062 to M.K.).

PY - 2011

Y1 - 2011

N2 - We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

AB - We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

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The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNa from Pyrococcus horikoshii OT3

Abstract

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