The Complete Amino Acid Sequence of Chitinase-B from the Leaves of Pokeweed (Phytolacca americana)

Miho Tanigawa; Takeshi Yamagami; Gunki Funatsu

doi:10.1271/bbb.59.841

The Complete Amino Acid Sequence of Chitinase-B from the Leaves of Pokeweed (Phytolacca americana)

Miho Tanigawa, Takeshi Yamagami, Gunki Funatsu

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 tryptic peptides derived from the reduced and S-carboxymethylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecular mass of 29, 473 Da. Six cysteine residues are linked by disulfide bonds between Cys20 and Cys67, Cys50 and Cys57, and Cys159 and Cys188. From 58-68% sequence homology of PLC-B with five class III chitinases, it was concluded that PLC-B is a basic class III chitinase.

Original language	English
Pages (from-to)	841-847
Number of pages	7
Journal	Bioscience, biotechnology, and biochemistry
Volume	59
Issue number	5
DOIs	https://doi.org/10.1271/bbb.59.841
Publication status	Published - 1995

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 tryptic peptides derived from the reduced and S-carboxymethylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecular mass of 29, 473 Da. Six cysteine residues are linked by disulfide bonds between Cys20 and Cys67, Cys50 and Cys57, and Cys159 and Cys188. From 58-68% sequence homology of PLC-B with five class III chitinases, it was concluded that PLC-B is a basic class III chitinase.",

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AB - The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 tryptic peptides derived from the reduced and S-carboxymethylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecular mass of 29, 473 Da. Six cysteine residues are linked by disulfide bonds between Cys20 and Cys67, Cys50 and Cys57, and Cys159 and Cys188. From 58-68% sequence homology of PLC-B with five class III chitinases, it was concluded that PLC-B is a basic class III chitinase.

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The Complete Amino Acid Sequence of Chitinase-B from the Leaves of Pokeweed (Phytolacca americana)

Abstract

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